Original Article

Inhibition growth of pomegranate seeds extract against streptococcus sanguis: the cause of recurrent aphthous stomatitis

Riani Setiadhi , Irna Sufiawati, Dewi Zakiawati, Nanan Nur’aeny, Wahyu Hidayat, Dani R. Firman

Riani Setiadhi
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University Bandung Indonesia. Email: riani.setiadhi@unpad.ac.id

Irna Sufiawati
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University Bandung Indonesia

Dewi Zakiawati
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University Bandung Indonesia

Nanan Nur’aeny
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University Bandung Indonesia

Wahyu Hidayat
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University Bandung Indonesia

Dani R. Firman
Department of Oral Biology, Faculty of Dentistry, Padjadjaran University Bandung Indonesia
Online First: April 01, 2017 | Cite this Article
Setiadhi, R., Sufiawati, I., Zakiawati, D., Nur’aeny, N., Hidayat, W., Firman, D. 2017. Inhibition growth of pomegranate seeds extract against streptococcus sanguis: the cause of recurrent aphthous stomatitis. Journal of Dentomaxillofacial Science 2(1): 7-12. DOI:10.15562/jdmfs.v2i1.452


Objective : Pomegranate (Punica granatum L.) seeds contain high of phytonutrients and phytochemicals, rich in polyphenol antioxidants namely tannins and flavonoids which also have antibacterial activity. Streptococcus sanguis is a bacterium known as one of the factors causing Recurrent Aphthous Stomatitis (RAS). To examine the potential antibacterial of Pomegranate seeds against Streptococcus sanguis.

Material anda Methods : In vitro study of Pomegranate seed were extracted with maceration method using 70% ethanol as the solvent to obtain stable extract, continued with phytochemical screening against phenolic, flavonoids, alkaloids, steroids, triterpenoid, saponins and tannins.  The extract was evaluated for Minimum Inhibitory Concentration and Minimum Bactericide Concentration against Streptococcus sanguis ATCC 10556, using microdiluted method through 96 wells microplate.

Results : Chlorhexidine was used as positive control while 70% ethanol was used as solvent as well as negative control. Phytochemical screening gave positive results for phenolics, flavonoids, steroids, saponins and tannins. Microdilution test showed the concentration of 500 ppm as MIC and MBC value at 2,000 ppm.

Conclusion : Pomegranate seeds extract have a growth inhibitory against Streptococcus sanguis with MIC value of 500 ppm and 2,000 ppm as MBC.

References

Jurenka, J. Therapeutic applications of Pomegrante (Punica granatum L.): A Review. Altern Med Rev 2008;13: 128-131.

Jozinete VP, Maria SVP, Fábio CS, et al. In vitro antibacterial and antiadherence effect of Punica Granatum linn extract upon dental Biofilm microorganisms. Rev Braz Farmacogn 2006;16: 88-93.

Greenberg M, Ulcerative, Vesicular, et al. Burket’s Oral Medicine. 10th ed. Spain: BC Decker; 2003. p. 63-65.

Field A, Lesley L, William RT. Tyldesley’s Oral Medicine. 5th ed. Oxford: Oxford University Press; 2004. p. 49-60.

Preeti L, Magesh KT, Rajkumar K, et al. Recurrent Aphthous Stomatitis. J Oral Maxillofac Pathol 2011;15: 252-256.

Scully C, Porter S. Orofacial disease. 1st ed. Edinburgh: Churchill Livingstone; 2003. p. 13-15.

Scully C. Oral and Maxillofacial medicine the basis of diagnosis and treatment. 2nd ed. Edinburgh: Churchill Livingstone Elsevier; 2008. p. 12.

Anibal PC, Peixoto ITA, Foglio MA, et al. Antifungal activity of the Ethanolic extracts of Punica Granatum L and evaluation of the Morphological and structural modifications of its compounds upon the cells of Candida sp. Braz J Microbiol 2013;44: 839-848.

Duman AD, Ozgen M, Dayisoylu KS, et al. Antimicrobial activity of six Pomegranate (Punica granatum L.) varieties and their relation to some of their Pomological and Phytonutrient characteristics. Molecules 2009;14: 1808-1817.

Egharevba, Omoregie H, Kunle et al. Phytochemical analysis and antimicrobial activity of Punica Granatum L. (fruit bark and leaves) N Y Sci J 2010;12: 3.

Abdollahzadeh Sh, Mashouf RY, Mortazavi H, et al. Antibacterial and antifungal activities of Punica Granatum Peel extracts against Oral Pathogens. J Dent (Tehran) 2011;8: 1-6.

Saravanakumar A. Evaluation of antibacterial activity Phenol and flavonoid contents of the Spesiapulnea flower extracts. Pak J Pharm Sci 2013;22: 283-286.

Karamian R, Ghasemlou F. Screening of total phenol and flavonoid content, antioxidant and antibacterial activities of the Methanolic extracts of three Silene species from Iran. IJACS 2013;5: 305-312.

Hanee S, Khan JA. Antibacterial properties of Punica granatum Peel. Int J Appl Biol Pharm 2011;2: 23-27.

Vasconcelos LCD, Sampaio FC, Sampaio MCC, et al. Minimum inhibitory concentration of adherence of Punica granatum Linn (Pomegranate) gel against S. Mutans, S. Mitis and C. Albicans. Braz Dent J 2006;17: 223-227.

Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001;48: 5-16.


No Supplementary Material available for this article.
Article Views      : 263
PDF Downloads : 141