Original Article

Effect of low intensity pulse ultrasound on the neutrophil function and epithelial thickness after skin excision in diabetes mellitus type 2 rats’ model (an immunohistochemistry study on matrix metalloproteinase-9)

Titis Laraswati , Cahya Y. Hasan, Bambang Dwirahardjo

Titis Laraswati
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia. Email: titislaraswati@yahoo.co.id

Cahya Y. Hasan
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia

Bambang Dwirahardjo
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia
Online First: April 01, 2021 | Cite this Article
Laraswati, T., Hasan, C., Dwirahardjo, B. 2021. Effect of low intensity pulse ultrasound on the neutrophil function and epithelial thickness after skin excision in diabetes mellitus type 2 rats’ model (an immunohistochemistry study on matrix metalloproteinase-9). Journal of Dentomaxillofacial Science 6(1): 54-60. DOI:10.15562/jdmfs.v6i1.1035


Objective: To determine the effect of Low Intensity Pulse Ultrasound (LIPUS) on the neutrophil function and epithelial thickness after skin excision in diabetes mellitus type 2 rats’ model (an Immunohistochemistry Study on Matrix Metalloproteinase-9).

Material and Methods: Thirty-six Sprague dawley rats were divided into six groups: DM2, DM4, DM7, DML2, DML4, and DML7, each of which consist of 6 rats. Rats that matched the inclusion criteria were made into diabetes mellitus type 2 by injecting Nicotinamide and Streptozotocin. In all rat’s excision wound was made with punch biopsy in the back area. DML group received LIPUS therapy in the wound area (frequency: 3 MHz, intensity: 0.5 W/cm2, duty cycle: 20%, duration: 3 minutes every day) for two days (DML2), four days (DML4) and seven days (DML7). In the wound tissue an immunohistochemical examination was performed with Polyclonal Antibody MMP-9 to observe MMP-9 expression and and Periodic Acid-Schiff staining to observe epithelial thickness.

Results: Two-Way ANOVA and Post-Hoc LSD test showed lower level of MMP-9 expressions in the DML group than in the DM group, whereas the epithelial thickness in the DML group was found higher than in the DM group. The Pearson test showed a correlation between the level of MMP-9 expressions and epithelial thickness (p=0.000; r=-0.785).

Conclusion: LIPUS therapy reduced MMP-9 expression and increases epithelial thickness.

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