Skip to main content Skip to main navigation menu Skip to site footer

Antibacterial activities of moringa olifiera freeze dried extract on staphylococcus aureus

  • Wulan Angestia ,
  • Valendriyani Ningrum ,
  • Tai L. Lee ,
  • Shih-Chieh Lee ,
  • Abu Bakar ,


Abstract

Objective: The aim of this study is to determine the antibacterial activities of M. oleifera freeze dried extract on Staphylococcus aureus. Material and Methods: The antibacterial activities using two methods, microdilution method and agar diffusion method. The minimum inhibitory concentration is determined. The measurement of absorbance in microdilution method using a spectrophotometer at length wave 590 nm. The inhibition zone is measured using scalimeter. Results: The results showed both microdilution method and agar diffusion method present the inhibition activity of M. oleifera freeze dried extract on staphylococcus aureus. Conclusion: The finding confirmed M. oleifera freeze dried extract as the promising antibacterial natural agent, particularly on inhibiting S. aureus strain.  
Keywords: Antibacterial freeze dried Staphylococcus aureus
Section

References

  1. Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: a review on nutritive importance and its medicinal application. Food Sci Hum Wellness 2016;2: 49-56.
  2. Thurber MD, Fahey JW. Adoption of moringa oleifera to combat under-nutrition viewed through the lens of the “diffusion of innovations” theory. Ecol Food Nutr 2009;48: 212-225.
  3. Inbathamizh L, Padmini E. Effect of geographical properties on the phytochemical composition and antioxidant potential of Moringa oleifera flowers. Bio Med Rx 2013;1: 239-247.
  4. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed 2013;3: 623-627.
  5. Vieira GHF, Mourão JA, Ângelo ÂM, et al. Antibacterial effect (in vitro) of moringa oleifera and annona muricata against gram positive and gram-negative bacteria. Rev Inst Med Trop Sao Paulo 2010;52: 129-132.
  6. Nepolean P, Anitha J, Renitta RE. Isolation, analysis and identification of phytochemicals of antimicrobial activity of Moringa oleifera Lamk. Current Biotica 2009;3: 33-39.
  7. Moyo. Antimicrobial activities of moringa oleifera lam leaf extracts. African J Biotechnol 2012;11: 2797-2802.
  8. Peixoto JRO, Silva GC, Costa RA, et al. In vitro antibacterial effect of aqueous and ethanolic moringa leaf extracts. Asian Pac J Trop Med 2011;4: 201-204.
  9. Doughari JH, Pukuma MS, De N. Antibacterial effects of balanites aegyptiaca L. Drel. and Moringa oleifera Lam. on Salmonella typhi. African J Biotechnol 2007;6: 2212-2215.
  10. Faizi S, Siddiqui BS, Saleem R, et al. Fully acetylated carbamate and hypotensive thiocarbamate glycosides from Moringa oleifera. Phytochem 1995;38: 957-663.
  11. Faizi S, Siddiqui BS, Saleem R, et al. Hypotensive constituents from the pods of Moringa oleifera. Planta Med 1998;64: 225-228.
  12. Guevara AP, Vargas C, Sakurai H, et al. An antitumor promoter from Moringa oleifera Lam. Mutat Res - Genet Toxicol Environ Mutagen 1999;440: 181-188.
  13. Suphachai C. Antioxidant and anticancer activities of moringa oleifera leaves. J Med Plants Res 2014;8: 318-325.
  14. Al-Asmari AK, Albalawi SM, Athar MT, et al. Moringa oleifera as an anti-cancer agent against breast and colorectal cancer cell lines. PLoS One 2015;10: 1-14.
  15. Khor KZ, Lim V, Moses EJ, et al. The in vitro and in vivo anticancer properties of moringa oleifera. Evidence-based Complement Altern Med 2018;2018: 1-14.
  16. Ghasi S, Nwobodo E, Ofili JO. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats. J Ethnopharmacol 2000;69: 21-25.
  17. Villarruel-López A, López-de LMDA, Vázquez-Paulino OD, et al. Effect of moringa oleifera consumption on diabetic rats. BMC Complement Altern Med. 2018;18: 1-10.
  18. Metwally FM, Rashad HM, Ahmed HH, et al. Molecular mechanisms of the anti-obesity potential effect of Moringa oleifera in the experimental model. Asian Pac J Trop Biomed 2017;7: 214-221.
  19. Elgamily H, Moussa A, Elboraey A, et al. Microbiological assessment of moringa oleifera extracts and its incorporation in novel dental remedies against some oral pathogens. Maced J Med Sci 2016;4: 585-590.
  20. Auwal M, Tijjani A, Sadiq M, et al. Antibacterial and haematological activity of Moringa oleifera aqueous seed extract in Wistar albino rats. Sokoto J Vet Sci 2013;11: 28-37.
  21. Abubakar I, Usman A. Phytochemical and antibacterial investigations of moringa (moringa oleifera) leaf extract on selected bacterial pathogens. J Microbiol Antimicrob 2016;8: 28-33.
  22. Van-Der-Watt E, Pretorius JC. Purification and identification of active antibacterial components in carpobrotus edulis L. J Ethnopharmacol 2001;76: 87-91.
  23. Clark AM, El?Feraly AS, Li W ?S. Antimicrobial activity of phenolic constituents of magnolia grandiflora L. J Pharm Sci 1981;70: 951-952.
  24. Mandal P, Sinha Babu SP, Mandal NC. Antimicrobial activity of saponins from Acacia auriculiformis. Fitoterapia 2005;76: 462-465.
  25. Akiyama H. Antibacterial action of several tannins against staphylococcus aureus. J Antimicrob Chemother 2001;48: 487-491.
  26. Chung KT, Wong TY, Wei CI, et al. Tannins and human health: a review. Crit Rev Food Sci Nutr 1998;38: 421-464.
  27. Kou X, Li B, Olayanju JB, et al. Nutraceutical or pharmacological potential of moringa oleifera lam. Nutr 2018;10: 343-345.
  28. Hamudeng AM, Serliawati. Effectiveness of antibacterial extract of coriander seeds (coriandrum sativum L.) against staphylococcus aureus. J Dentomaxillofac Sci 2019;4: 71-74.
  29. Klancnik A, Piskernik S, Jersek B, Mozina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods. 2010;81: 121-126.

How to Cite

Angestia, W., Ningrum, V., Lee, T. L., Lee, S.-C., & Bakar, A. (2020). Antibacterial activities of moringa olifiera freeze dried extract on staphylococcus aureus. Journal of Dentomaxillofacial Science, 5(3), 154–157. https://doi.org/10.15562/jdmfs.v5i2.1043
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
403

Total
1257

Share