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Bioactive Potential of Edel Cocoa Bean (Theobroma Cacao L) from Kedaton Jember: Cytotoxicity and Antioxidants Evaluation

Abstract

Objective: Kedaton, a small village located in Jember is well known as one of the largest edel cocoa producers in the world. The cocoa beans contain nutrients and numerous beneficial properties, one of them is flavonoids, that work as antioxidants that can be utilized in dentistry, particularly orthodontics. The use of plants for the treatment must be ensured as safe without any toxic properties. Material and Methods: This study use DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method to evaluate antioxidant properties and MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to assess the fibroblast cell activity. Results: The fermented edel cocoa bean extract exhibited the IC50 value of 33.97 ppm and the unfermented edel cocoa bean extract that had the IC50 value of 9.56 ppm.  Both of them have very strong antioxidant activity. Fermented edel cocoa bean extract in concentrations of 1.56%, 3.125%, 6.25%, and 12.5%; as well as unfermented edel cocoa bean extract in concentrations of 1.56% and 3.125% did not have cytotoxic effects to fibroblasts. Conclusion: The unfermented edel cocoa bean extracts have higher antioxidant activity compared to fermented ones. The fermented edel cocoa bean extracts did not have cytotoxic effect, meanwhile the unfermented edel cocoa bean extracts in concentrations of 6.25% and 12.5% have cytotoxic effect to fibroblasts. 
Section

References

  1. Rosniati K, Kalsum. Processing of cocoa powder from fermented and non-fermented cocoa beans for functional food preparations. Jurnal Industri Hasil Perkebunan 2018;13(2): 107-116.
  2. Ali B. Analisis sistem pendukung keputusan pemilihan bibit kakao menggunakan metode AHP. d'ComPutarE: Jurnal Ilmiah Information Technology 2020; 9(2): 8-17.
  3. Haliza W, Purwani EY, Fardiaz D, Suhartono MT. Kakao fermentasi: the release of bioactive peptides and their health benefits. Perspektif 2020;18(2): 104-119.
  4. Cambrai AE, Marchioni, Julien-David D, Marcic C. Discrimination of cocoa bean origin by chocolate polyphenol chromatographic analysis and chemometrics. Food analytical methods 2017;10(6): 1991-2000.
  5. Gu F, Tan L, Wu Hu,et al. Comparison of Cocoa Beans from China, Indonesia and Papua New Guinea. Foods 2013;2: 183-197.
  6. Adabe K, Ngo-Samnick L. Cocoa: Production and processing. CTA; 2014.
  7. Sudibyo A. Effect of processing techniques on flavour and characteristics of cocoa processed and chocolate products. Jurnal Industri Hasil Perkebunan 2017;12(1): 1-13.
  8. Rahmawati F. Fortification of Moringa oil (Moringa oliifera) powder with milk powder and concentration of Cinnamon (Cinnamomun burmani) against dark chocolate. Doctoral dissertation Fakultas Teknik Unpas 2016.
  9. Ariyanti M, Wahyuni W. Flavonoids and total phenol content in fermented cocoa powder. In Seminar Nasional Hasil Penelitian & Pengabdian Kepada Masyarakat (SNP2M) 2019; 76-79.
  10. Urba?ska B, Kowalska J. Comparison of the total polyphenol content and antioxidant activity of chocolate obtained from roasted and unroasted cocoa beans from difffurbaerent regions of the World. Antioxidants 2019;8(8): 283.
  11. Utami RR, Armunanto R, Rahardjo S, et al. Effects of cocoa bean (Theobroma cacao L.) fermentation on phenolic content, antioxidant activity and functional group of cocoa bean shell. Pakistan Journal of Nutrition 2016;15: 948-953.
  12. Papas AM. Antioxidant status, diet, nutrition, and health. CRC press 2019; Table 4.
  13. Yuslianti ER. Introduction to free radicals and antioxidants. Deepublish. 2018;1.
  14. Al-Fa’izah Z. Effectiveness of cocoa bean extract gel (Theobroma cacao l.) extract gel on the number of fibroblast cells in post-tooth extraction sockets in male wistar rats. Digital Repository Universitas Jember 2018; 1- 50.
  15. Fitriani F, Soetojo A, Subiwahjudi A. Cytotoxicity of cocoa bark extract (Theobroma cacao) against bhk-21 fibroblast cell culture. Conservative Dentistry Journal 2019;9(1): 54-65.
  16. Aprilia V, Meizarini A, Agustantina TH. Cytotoxicity of combination of zinc-oxide and Curcuma longa extract against human gingival fibroblasts. Jurnal Material Kedokteran Gigi 2019;8(1): 1-6.
  17. Tristantini D, Ismawati A, Pradana BT, et al. Testing of antioxidant activity using the dpph method on tanjung leaves (mimusops elengi l). In Seminar Nasional Teknik Kimia Kejuangan 2016;1.
  18. Nuraeni F, Sembiring SBB. Antioxidant activity and identification of compounds from lingzhi mushroom extract (Ganoderma lucidum) using liquid chromatography-mass spectrometry (Lc-Ms). In Prosiding Seminar Nasional & Internasional 2018;1(1).
  19. Puspitasari E, Ningsih IY. Antioxidant capacity of salak fruit extract (Salacca zalacca (Gaertn.) Voss) sugar variant using DPPH radical capture method.. Pharmacy: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 2016; 13(1): 116-126.
  20. Katrin K, Bendra A. Antioxidant activity of Premna oblongata Miq. extracts, fractions and chemical compound groups. Pharmaceutical Sciences and Research (PSR) 2017;2(1): 21-31.
  21. Rachmawati D, Alsalem IW, Bontkes HJ, et al. Innate stimulatory capacity of high molecular weight transition metals Au (gold) and Hg (mercury). Toxicology in Vitro 2015; 29(2): 363-369.
  22. Armanda F, Nahzi MYI, Budiarti LY. The effectiveness of bacterial inhibition of bacterial onion Extract standardized by flavonoids against Enterococcus faecalis (In Vitro). Dentino 2017;2(2): 183-187.
  23. Suhendra CP, Widarta IWR, Wiadnyani AAIS. The effect of ethanol concentration on the antioxidant activity of grass roots extract (Imperata cylindrica (L) Beauv.) In extraction using ultrasonic waves. Jurnal Ilmu dan Teknologi Pangan (ITEPA) 2019;8(1): 27-35.
  24. Oracz J, Nebesny E. Antioxidant properties of cocoa beans (Theobroma cacao L.): influence of cultivar and roasting conditions. International journal of food properties. 2016;19(6): 1242-1258.
  25. Scapagnini G, Davinelli S, Di Renzo L, et al. Cocoa bioactive compounds: significance and potential for the maintenance of skin health. Nutrients 2014;6(8): 3202-3213.
  26. Purgiyanti P, Purba AV, Winarno H. Determination of total phenol levels and combination antioxidant activity test of herba gotu kola extract (Centella asiatica L. Urban) and mahkota dewa fruit (Phaleria macrocarpa (Scheff.) Boerl.). Parapemikir: Jurnal Ilmiah Farmasi 2019;8(2): 40-45.
  27. Saxena M, Nema R, Gupta A. Phytochemistry of medicinal plants. Joural of Pharmacognosy and Phytochemistry 2013;12(1): 168-169.
  28. Nararya SA. Toxicity test of Moringa oleifera leaves against gingival fibroblasts using MTT assay. Jurnal Biosains Pascasarjana 2015;17(1): 52-58.
  29. Nugroho SW, Rukmo M, Prasetyo EA, Yuanita T. Antibacterials of cocoa fruit skin extract (Theobroma cacao) 6.25% and NaOCl 2.5% against Streptococcus sanguinis bacteria. Conservative Dentistry Journal 2019;9(1): 19-21.
  30. Dwijatmoko MI, Nurtama B, Yuliana ND, Misnawi M. Characterization of polyphenols from various Cocoa (Theobroma cacao L.) clones during fermentation. Pelita Perkebunan a Coffee and Cocoa Research Journal 2018;34(2): 104-112.

How to Cite

Sutjiatu, R., Devi, L. S. ., Prijatmoko, D. ., Herniyati, H., Joealijanto, R. ., Sulistyani, E., Safitri, B. P. ., & Fitriyani, N. (2022). Bioactive Potential of Edel Cocoa Bean (Theobroma Cacao L) from Kedaton Jember: Cytotoxicity and Antioxidants Evaluation. Journal of Dentomaxillofacial Science, 7(1), 25–29. https://doi.org/10.15562/jdmfs.v5i2.1068

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