Original Article

Acceleration of granulation tissue using myrmecodiapendens extract induction during wound healing tooth extraction process (experimental research on caviacobaya)

Efa Ismardianita , Wenny Rosalina

Efa Ismardianita
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Baiturrahmah, Padang, Indonesia. Email: efa_ismardianita@yahoo.co.id

Wenny Rosalina
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Baiturrahmah, Padang, Indonesia
Online First: August 01, 2020 | Cite this Article
Ismardianita, E., Rosalina, W. 2020. Acceleration of granulation tissue using myrmecodiapendens extract induction during wound healing tooth extraction process (experimental research on caviacobaya). Journal of Dentomaxillofacial Science 5(2): 124-128. DOI:10.15562/jdmfs.v5i2.1071

Objective:To identify the effect of myrmecodiapendens extract 20% on granulation tissue post-tooth extraction.

Material and Methods: Twenty-four caviacobaya divided into two groups: the control group and treatment group. Lower left incisor tooth subjects were extracted and the entire socket filled with CMC 0.5% in the control group and myrmecodiapendens extract 20% in the treatment group, 0.1 CC in each subject. On days 3, 7, 14, and 21 subjects were determined, subsequently, socket tissues were stained with Haematoxylin Eosin (HE) technique. Angiogenesis, fibroblast, and macrophage were used to evaluated granulation tissue on wound healing post-tooth extraction. Data were analyzed with the Kruskal-Wallis test to see differences between the control group and treatment group with a level of significant p <0.05.

Results: This study indicates significant differences in angiogenesis, fibroblast, and macrophage between control group and treatment group (p <0.05).

Conclusion: This study confirmed the effect of myrmecodiapendens extract 20% increase angiogenesis, fibroblast, and macrophage, which is can improve wound healing process post-tooth extraction.


Kareem JJ. Post operative complications associated with

non-surgical tooth extraction. MDJ 2008;5: 104-113.

Schmidlin PR, Imfeld P, Sahrmann A, et al. Effect of shorttime povidone-iodine application on osteoblast proliferation

and differentiation. Open Dentist J 2009;3: 208-212.

Politis C, Joseph S, Reinhilde J, et al. Wound healing

problems in the mouth. Front Physiol 2016;7: 1-13.

Rowan MP, Cancio LC, Elster EA, et al. Burn wound

healing and treatment: review and advancements. Critical

Car 2015;19: 143.

Honnegowda TM, Pramod K, Echalarasa GPU, et al. Role

of angiogenesis in acute and chronic wound healing. Plast

Aesthet Res 2015;2: 243-249.

Tonnesen MG, Feng X, Clark RAF. Angiogenesis in wound

helaing. New York: Department of Dermatology, Health

Sciences Center; 2000. p. 40-46.

Supriatno DR. antitumor activity of papuas’s myrmecodiapendens in human oral tongue squamous cell carcinoma

cell line through induction of cyclindependent kinase

inhibitor p27Kp1 and suppression of cyclin E. J of Cancer

Res and Ther 2014;2: 48-53.

Soeksmanto A, Subroto M, Wijaya H, et al. Anticancer

activity test for extracts of sarangsemut (myrmecodiapendens) to HeLa and MCM-B2 cells. Pak J Biol Sci 2010;13:


Roslizawaty, Budiman H, Laila H, et al. The effect ethanolic

extraxt of ant plant (myrmecodia sp.) on histopatological

changes in the kidney of hiperurisemic male mice (mus

musculus). J Medika Veterinaria 2013;7: 116-120.

Gonzales AC, Costa TF, Andrade ZA, et al. Wound

healing-a literature review. An Bras Dermatol 2016;91:


Hashemi SA, Madani SA, Abediankenari S. The Review

on properties of Aloe vera in healing of cutaneouswounds.

Biomed Res Int 2015: 1-6.

Agarwal PK, Singh A, Gaurav K, et al. Evaluation of

wound healing activity of extract plantae banana (M. sapientum var. paradisiaca) in rats. Indian J Exp Biol 2009;47:


Kresnoaadi U. The increasing of fibroblast growth factor 2,

osteocalsin and osteoblast due to the induction of

combination of Aloe veraand 2% xenograft concelous

bovine. Dent J 2012;45: 228-232.

Silva SS, EG Popa, ME Gomes, et al. An Investigation of

the potential application of chitosan/aloe-based

membranes for regenerative medicin. ActaBiomat. 2013;9:


Diegelmann RF, Evans MC. Wound healing: am overview

of acute, fibrotic and delayed healing. Front Biosci 2004;9:


Mukherjee A, Roychowdhury B. The in vitro propagation

of aloe vera sp. TIG Res J 2008;1: 116-119.

Sobazo SE, Soto PG, Valdes RC, et al. Applied biological

and physicochemical activity of isoquinoline alkaloids:

oxoisoaporphine and boldine. Molec 2012;17:


Fetse JP, James OK, Evans D, et al. Wound healing activity

of total alkaloidal extract of the root bark of Aistonia

boonei (Apocynacea). British J Pharm Res Sci Dom

;2: 2642-2652.

Ismardianita E, Widyawati, Elianora D, et al. The

effectiveness methanol extract clausena excavate on

number of fibroblast and densityof collagen fibers after

tooth extraction. J Dentomaxillofac Sci 2019;4: 170-175.

No Supplementary Material available for this article.
Article Views      : 102
PDF Downloads : 58