Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement
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Aug 1, 2020
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Abstract
Objective: The aim of this study was to analyze the effect of moringa leaf extract administration at 5%, 10%, and 20% concentration toward osteoblast and osteoclast cell count in tension area of Cavia cobaya tooth movement. Matrial and Methods: This study was an experimental research with post test only control group design conducted on Cavia Cobaya. The samples were divided into 4 groups, namely control group (given orthodontic forces), P1 group (given orthodontic forces and Moringa oleifera extract at 5% concentration), P2 group (given orthodontic forces and Moringa oleifera extract at 10% concentration) and P3 group (given orthodontic forces and Moringa oleifera extract at 20% concentration). The samples were sacrificed in day 15 and the tissue were collected for histological examination to examine osteoblast and osteoclast cell count.Results: The Anova test showed a significant difference of osteoblast and osteoclast cell count among groups (p<0.05).Conclusion: Moringa leaves extract administration at 20% concentration for 7 days shows higher number of osteoblast and osteoclast compared to control group, 5% and 10% extract group.
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How to Cite
Syarif, R. D., Kusumaningsih, T., & Arundina, I. (2020). Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement. Journal of Dentomaxillofacial Science, 5(2), 98–102. https://doi.org/10.15562/jdmfs.v5i2.1081
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mesenchymal stem cells. Int Conf Adv Agric Biol Ecol Sci
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in compression and tension sides during orthodontic
movement. Cytokine 2008;42: 330-335.
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of antioxidants. Clin Cases Miner Bone Metab 2017;14:
209-216.
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Bioactive components in moringa oleifera leaves protect
against chronic disease. Antioxidants 2017;6: 1-13.
13. Dudaric L, Fuzinac-Smojver A, Muhvic D, et al. The role
of polyphenols on bone metabolism in osteoporosis. Food
Res Int 2015;77: 290-298.
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1183-1226.
15. Pang JL, RicuperoDA, Huang S, et al. Differential activity
of kaempferol and quercetin inattenuating tumor necrosis
factor receptor familysignaling in bone cells. Biochemical
Pharmacology 2006;71: 818-826.
16. Srivastava S, Bankar R, Roy P. Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated
mouse bone marrow derived mesenchymal stem cells.
Phytomedicine 2013;20: 683-690.
17. Horcajada M-N, Offord E. Naturally plant-derived
compounds: role in bone anabolism. Curr Mol
Pharmacol 2012;5: 205-218.
18. Wattel A, Kamel S, Prouillet C, et al. Flavonoid Quercetin
decreases osteoclastic differentiation induced by RANKL
via a mechanism involving NFκB and AP-1. J Cell Biochem
2004;92: 285-295.
19. Ismardianita E, Nasrul E, Yanwirasti, et al. Effect of ethanol
extract of myrmecodia pendens on TGF-β1 expression
and osteoblast cells after tooth extraction (experimental
research on cavia cobaya). J Dentomaxillofac Sci 2017;2:
150-154.
20. Kajiya M, Giro G, Taubman MA, et al. Role of Periodontal
Pathogenic Bacteria in RANKL-mediated Bone
Destruction in Periodontal Disease. J Oral Microbiol
2010;2:1-17.
formation in orthodontic tooth movement via modulation
of the GSK-3β/β-catenin signaling pathway. J Mol Histol
2018;49:75-84.
2. Nagarajan D, Usha K, Rao GU, et al. Biomarkers in
orthodontics. Int J Oral Heal Med Res 2015;2: 88-90.
3. Ariffin SHZ, Yamamoto Z, Abidin IZZ, et al. Cellular and
molecular changes in orthodontic tooth movement. Sci
World J 2011;11: 1788-1803.
4. Rucci N. Molecular biology of bone remodelling. Clin
Cases Miner Bone Metab 2008;5: 49-56.
5. Baloul SS. Osteoclastogenesis and osteogenesis during
tooth movement. Front Oral Biol 2016;18: 75-79.
6. Dubey DK, Dora J, Kumar A, et al. A multipurpose treemoringa oleifera. J Pharm Chem Sci 2013;2: 415-423.
7. Yokomizo A, Moriwaki M. Effects of uptake of flavonoids
on oxidative stress induced by hydrogen peroxide in
human intestinal caco-2 cells. Biosci, Biotechnol, Biochem
2006;70: 1317-1324.
8. Zhang DW, Cheng Y, Wang NL, et al. Effects of total flavonoids and flavonol glycosides from epimedium koreanum
nakai on the proliferation and differentiation of primary
osteoblasts. Phytomedicine 2008;15: 55-61.
9. Marupanthorn K, Kedpanyapong W. The effects of moringa oleifera lam. leaves extract on osteogenic
differentiation of porcine bone marrow derived
mesenchymal stem cells. Int Conf Adv Agric Biol Ecol Sci
2016: 29-32.
10. Garlet TP, Coelho U, Repeke CE, et al. Differential
expression of osteoblast and osteoclast chemmoatractants
in compression and tension sides during orthodontic
movement. Cytokine 2008;42: 330-335.
11. Domazetovic V. Oxidative Stress in bone remodeling: role
of antioxidants. Clin Cases Miner Bone Metab 2017;14:
209-216.
12. Vergara-Jimenez M, Almatrafi MM, Fernandez M.
Bioactive components in moringa oleifera leaves protect
against chronic disease. Antioxidants 2017;6: 1-13.
13. Dudaric L, Fuzinac-Smojver A, Muhvic D, et al. The role
of polyphenols on bone metabolism in osteoporosis. Food
Res Int 2015;77: 290-298.
14. Torre E. Molecular signaling mechanisms behind polyphenol-induced bone anabolism. Phytochem Rev 2017;16:
1183-1226.
15. Pang JL, RicuperoDA, Huang S, et al. Differential activity
of kaempferol and quercetin inattenuating tumor necrosis
factor receptor familysignaling in bone cells. Biochemical
Pharmacology 2006;71: 818-826.
16. Srivastava S, Bankar R, Roy P. Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated
mouse bone marrow derived mesenchymal stem cells.
Phytomedicine 2013;20: 683-690.
17. Horcajada M-N, Offord E. Naturally plant-derived
compounds: role in bone anabolism. Curr Mol
Pharmacol 2012;5: 205-218.
18. Wattel A, Kamel S, Prouillet C, et al. Flavonoid Quercetin
decreases osteoclastic differentiation induced by RANKL
via a mechanism involving NFκB and AP-1. J Cell Biochem
2004;92: 285-295.
19. Ismardianita E, Nasrul E, Yanwirasti, et al. Effect of ethanol
extract of myrmecodia pendens on TGF-β1 expression
and osteoblast cells after tooth extraction (experimental
research on cavia cobaya). J Dentomaxillofac Sci 2017;2:
150-154.
20. Kajiya M, Giro G, Taubman MA, et al. Role of Periodontal
Pathogenic Bacteria in RANKL-mediated Bone
Destruction in Periodontal Disease. J Oral Microbiol
2010;2:1-17.