Skip to main content Skip to main navigation menu Skip to site footer

Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement



Abstract

Objective: The aim of this study was to analyze the effect of moringa leaf extract administration at 5%, 10%, and 20% concentration toward osteoblast and osteoclast cell count in tension area of Cavia cobaya tooth movement. Matrial and Methods: This study was an experimental research with post test only control group design conducted on Cavia Cobaya. The samples were divided into 4 groups, namely control group (given orthodontic forces), P1 group (given orthodontic forces and Moringa oleifera extract at 5% concentration), P2 group (given orthodontic forces and Moringa oleifera extract at 10% concentration) and P3 group (given orthodontic forces and Moringa oleifera extract at 20% concentration). The samples were sacrificed in day 15 and the tissue were collected for histological examination to examine osteoblast and osteoclast cell count.Results: The Anova test showed a significant difference of osteoblast and osteoclast cell count among groups (p<0.05).Conclusion: Moringa leaves extract administration at 20% concentration for 7 days shows higher number of osteoblast and osteoclast compared to control group, 5% and 10% extract group.
Keywords: Moringa oleifera Orthodontic tooth movement Osteoblast Osteoclast Osteogenesis
Section

References

  1. Mao Y, Wang L, Zhu Y, et al. Tension force-induced bone
  2. formation in orthodontic tooth movement via modulation
  3. of the GSK-3β/β-catenin signaling pathway. J Mol Histol
  4. ;49:75-84.
  5. Nagarajan D, Usha K, Rao GU, et al. Biomarkers in
  6. orthodontics. Int J Oral Heal Med Res 2015;2: 88-90.
  7. Ariffin SHZ, Yamamoto Z, Abidin IZZ, et al. Cellular and
  8. molecular changes in orthodontic tooth movement. Sci
  9. World J 2011;11: 1788-1803.
  10. Rucci N. Molecular biology of bone remodelling. Clin
  11. Cases Miner Bone Metab 2008;5: 49-56.
  12. Baloul SS. Osteoclastogenesis and osteogenesis during
  13. tooth movement. Front Oral Biol 2016;18: 75-79.
  14. Dubey DK, Dora J, Kumar A, et al. A multipurpose treemoringa oleifera. J Pharm Chem Sci 2013;2: 415-423.
  15. Yokomizo A, Moriwaki M. Effects of uptake of flavonoids
  16. on oxidative stress induced by hydrogen peroxide in
  17. human intestinal caco-2 cells. Biosci, Biotechnol, Biochem
  18. ;70: 1317-1324.
  19. Zhang DW, Cheng Y, Wang NL, et al. Effects of total flavonoids and flavonol glycosides from epimedium koreanum
  20. nakai on the proliferation and differentiation of primary
  21. osteoblasts. Phytomedicine 2008;15: 55-61.
  22. Marupanthorn K, Kedpanyapong W. The effects of moringa oleifera lam. leaves extract on osteogenic
  23. differentiation of porcine bone marrow derived
  24. mesenchymal stem cells. Int Conf Adv Agric Biol Ecol Sci
  25. : 29-32.
  26. Garlet TP, Coelho U, Repeke CE, et al. Differential
  27. expression of osteoblast and osteoclast chemmoatractants
  28. in compression and tension sides during orthodontic
  29. movement. Cytokine 2008;42: 330-335.
  30. Domazetovic V. Oxidative Stress in bone remodeling: role
  31. of antioxidants. Clin Cases Miner Bone Metab 2017;14:
  32. -216.
  33. Vergara-Jimenez M, Almatrafi MM, Fernandez M.
  34. Bioactive components in moringa oleifera leaves protect
  35. against chronic disease. Antioxidants 2017;6: 1-13.
  36. Dudaric L, Fuzinac-Smojver A, Muhvic D, et al. The role
  37. of polyphenols on bone metabolism in osteoporosis. Food
  38. Res Int 2015;77: 290-298.
  39. Torre E. Molecular signaling mechanisms behind polyphenol-induced bone anabolism. Phytochem Rev 2017;16:
  40. -1226.
  41. Pang JL, RicuperoDA, Huang S, et al. Differential activity
  42. of kaempferol and quercetin inattenuating tumor necrosis
  43. factor receptor familysignaling in bone cells. Biochemical
  44. Pharmacology 2006;71: 818-826.
  45. Srivastava S, Bankar R, Roy P. Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated
  46. mouse bone marrow derived mesenchymal stem cells.
  47. Phytomedicine 2013;20: 683-690.
  48. Horcajada M-N, Offord E. Naturally plant-derived
  49. compounds: role in bone anabolism. Curr Mol
  50. Pharmacol 2012;5: 205-218.
  51. Wattel A, Kamel S, Prouillet C, et al. Flavonoid Quercetin
  52. decreases osteoclastic differentiation induced by RANKL
  53. via a mechanism involving NFκB and AP-1. J Cell Biochem
  54. ;92: 285-295.
  55. Ismardianita E, Nasrul E, Yanwirasti, et al. Effect of ethanol
  56. extract of myrmecodia pendens on TGF-β1 expression
  57. and osteoblast cells after tooth extraction (experimental
  58. research on cavia cobaya). J Dentomaxillofac Sci 2017;2:
  59. -154.
  60. Kajiya M, Giro G, Taubman MA, et al. Role of Periodontal
  61. Pathogenic Bacteria in RANKL-mediated Bone
  62. Destruction in Periodontal Disease. J Oral Microbiol
  63. ;2:1-17.

How to Cite

Syarif, R. D., Kusumaningsih, T., & Arundina, I. (2020). Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement. Journal of Dentomaxillofacial Science, 5(2), 98–102. https://doi.org/10.15562/jdmfs.v5i2.1081
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
370

Total
589

Share

Search Panel

Rika D. Syarif
Google Scholar
Pubmed
JDMFS Journal

Tuti Kusumaningsih
Google Scholar
Pubmed
JDMFS Journal

Ira Arundina
Google Scholar
Pubmed
JDMFS Journal