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LOCAL ANAESTHETIC INHIBITION OF LIDOCAINE ON THE GROWTH OF STREPTOCOCCUS MUTANS IN VITRO

  • Gostry A. Dohude ,
  • Mulyani Rahmah ,

Abstract

AbstractAim : This study aims to determine the effectiveness of lidocaine of local anaesthetic agent in inhibiting the growth of Streptococcus mutans bacteria.Material and Methods : This type of research is a laboratory experimental with a post test only control group design. Microorganism used was Streptococcus mutans and cultured at the Microbiology Laboratory of the Pharmacy, Universitas Sumatera Utara. The local anaesthetic tested was a solution of lidocaine diluted with NaCl 0,9% to 0,5%, 1%, and  2%. The antibacterial activity test was carried out using diffusion method by dripping 25 µl of lidocaine (0,5%, 1%, 2%), positive control (amoxicillin), and negative control (NaCl 0,9%) on disc paper, then placed on nutrient agar, and incubated. After 24 hours, the inhibition zone formed around the disc paper was observed.Results : Lidocaine showed antibacterial activity with the inhibition zone of 0,5%, 1%, 2% concentration was 6,98 mm, 7,66 mm, 8,36 mm. Then the data was analyzed using the One-way ANOVA test. From these results, there was a significant difference in the mean of the inhibitin zone (p < 0,05) between 0,5%, 1%, and 2% lidocaine, positive control, and negative control.Conclusion : Lidocaine has antibacterial activity against Streptococcus mutans bacteria with an effective concentration of 2%.
Section

References

  1. Yumoto H, Hirota K, Hirao K, Ninomiya M, Murakami K, Fujii H, et al. The pathogenic factors from oral Streptococci for systemic diseases. Int J Mol Sci 2019: 1-18.
  2. Muharammy F, Machmud R, Nelis S. Perbedaan daya hambat anestesi lokal lidocaine 2% dan articaine 4% terhadap pertumbuhan bakteri Porphyromonas gingivalis secara in vitro. Andalas Dent J 2017; 4(2): 89-97.
  3. Weinberg L, Peake B, Tan C, Nikfarjam M. Pharmacokinetics and pharmacodynamics of lignocaine: A review. World J Anesthesiol 2015; 4(2): 17-29.
  4. Ikhsan M, Mariati NW, Mintjelungan C. Gambaran penggunaan bahan anestesi lokal untuk pencabutan gigi tetap oleh dokter gigi di kota Manado. e-Gigi 2013; 1(2).
  5. Chan P, Uchizono JA. Pharmacokinetics and pharmacodynamics of anesthetics. In: Kaye AD, Kaye AM, Urman RD. Eds. Essentials of pharmacology for anesthesia, pain medicine, and critical care. New York: Springer, 2015: 3-48.
  6. Pelz K, Wiedmann-Al-Ahmad M, Bogdan C, Otten JE. Analysis of the antimicrobial activity of local anesthetics used for dental analgesia. J Med Microbiol 2008; 57: 88-94.
  7. Kesici S, Demirci M, Kesici U. Antibacterial effects of lidocaine and adrenaline. Int Wound J 2019: 1-5.
  8. Davis WW, Stout TR. Disc plate method of microbiological antibiotics assay. I. Factors influencing variability and error. Appl Microbiol 1971; 22(4): 659-65.
  9. Balouiri M, Sadiki M, Ibrisouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J of Pharm Analysis 2016; 6: 71-9.
  10. Kaewijaranai T, Srisatjaluk RL, Sakdajeyont W, Pairuchvej V, Wongsirichat N. The efficiency of topical anesthetics as antimicrobial agents: A review of use in dentistry. J Dent Anesth Pain Med 2018; 18(4): 223-33.
  11. Jhonson SM, John BES, Dine AP. Local anesthetics as antimicrobial agents: A review. Surg Infect 2008; 9: 205-13.
  12. Razavi BM, Bazzaz BSF. A review and new insights to antimicrobial action of local anesthetics. Eur J Clin Microbiol Infect Dis 2019: 1-12.
  13. Xu H, Zhang L, Arita H, Hanaoka K. Antimicrobial activity of local anesthetics. Pain Res 2003; 18: 19-24.

How to Cite

Dohude, G. A., & Rahmah, M. . (2022). LOCAL ANAESTHETIC INHIBITION OF LIDOCAINE ON THE GROWTH OF STREPTOCOCCUS MUTANS IN VITRO. Journal of Dentomaxillofacial Science, 7(2). https://doi.org/10.15562/jdmfs.v7i2.1103

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