Original Article

Cytotoxicity of 5% ethanol extracts of moringa oleifera leaf as an alternative of root canal irrigant to fibroblast BHK-21 cell culture

Juni J. Nugroho , Aries C. Trilaksana, Nurhayaty Natsir, Christine A. Rovani, Asrianti Asrianti, Noor Hikmah

Juni J. Nugroho
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia. Email: jektijuni@yahoo.co.id

Aries C. Trilaksana
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia

Nurhayaty Natsir
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia

Christine A. Rovani
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia

Asrianti Asrianti
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia

Noor Hikmah
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia
Online First: April 01, 2021 | Cite this Article
Nugroho, J., Trilaksana, A., Natsir, N., Rovani, C., Asrianti, A., Hikmah, N. 2021. Cytotoxicity of 5% ethanol extracts of moringa oleifera leaf as an alternative of root canal irrigant to fibroblast BHK-21 cell culture. Journal of Dentomaxillofacial Science 6(1): 39-41. DOI:10.15562/jdmfs.v6i1.1105


Objective: The purpose of this study was to determine the cytotoxicity of 5% ethanol extracts of Moringa oleifera leaf based on the percentage of fibroblast cell life after exposure to 5% ethanol extracts of Moringa oleifera leaf.

Material and Methods: This study was an experimental laboratory with posttest only control group design. The extract was macerated using ethanol. Toxicity tested using the MTT Assay method. The fibroblast cells distributed in 96 well microplate were divided into 5 treatment groups. Group I as a negative control containing culture media, group II as a positive control containing cells in culture media, group III 5% ethanol extracts of Moringa leaf, group IV 2% chlorhexidine solution and group V 2.5% NaOCl solution. The percentage of cell life is calculated from the optical density value of the ELISA reader. Data were analyzed using the Kruskal Wallis test followed by the Post Hoc test using the Mann Whitney test.

Results: The results showed the percentage of life of fibroblast cells after exposure to 5% ethanol extracts of Moringa oleifera leaf = 33.31%, 2.5% NaOCl = 28.89% and 2% chlorhexidine = 39.37% (p = 0.000, p < 0.05).

Conclusion: 5% ethanol extracts of Moringa oleifera and 2% chlorhexidine were moderately toxic to fibroblast cells while 2.5% NaOCl was strongly toxic (p = 0.004, p <0.05).

References

Trilaksana AC, Saraswati A. Efficacy of green tea leaf extract (camellia sinensis) with NaOCl 2.5% against enterococcus faecalis as an alternative solution for root canal irrigation. J Dentomaxillofac Sci 2017;1: 58-62.

Bajrami D, Hoxha V, Gotduysus O, et al. Cytotoxic effect of endodontic irrigants in vitro. Med Sci Basic Res 2014;20: 22-26.

Wahyuniwati, Nugroho JJ, Trilaksana AC, et al. Microhardness characteristics values of root canal dentin after application with different types of EDTA. J Dentomaxillofac Sci 2016;1: 49-52.

Mirhadi H, Azar MR, Abbaszadegan A, et al. Cytotoxicity of chlorhexidine-hydrogen peroxide combination in different concentrations on cultured human periodontal ligament fibroblasts. Dent Res J 2014;11: 645-648.

Alagl AS, Bedi S, Almas K. Phytosolutions for enterococcus faecalis in endodontics: an update. OHDM 2016;15: 332-335.

Mistry KS, Sanghvi Z, Parmar G, et al. Comparative evaluation of antimicrobial activity of herbal extracts with 5.25% sodium hypochlorite against multispecies dentinal biofilm. Saudi Endod J 2016;6: 71-76.

Goncalves LS, Rodrigues RCV, Andrade JCVA, et al. The effect of sodium hypochlorite and chlorhexidine as irrigant solutions for root canal disinfection: a systematic review of clinical trials. J Endod 2016: 1-6.

Mattulada IK. The choice of intracanal medicament between visits is rational. Dentofasial J 2010;9: 63-69. (In Indonesia)

Tanumihardja M. Root canal Irrigation Solution. Dentofasial J 2010;9: 108-115. (In Indonsia)

Mathew T, Shetty A, Hegde MN. Comparison of antimicrobial activities of moringa oleifera leaf, propolis, 2% clorhexidine gluconate and mtad on e. faecalis – an in-vitro study. RJPBC 2014;5: 163-173.

Anwar S, Yulianti E, Hakim A, et al. Toxicity test of aquades extract (room temperature) and hot distilled water (700C) Moringa oleifera Lam leaves against shrimp larvae (artemia salina leach). Alchemy 2014;1: 84-92. (In Indonesia)

Tejas GH, Umang JH, Payal BN, et al. A panoramic view on pharmacognostic, pharmacological, nutritional, therapeutic, and prop[hylactic, values of Moringa oleifera lam. IRJP 2012;3: 1-7.

Pandey A, Pandey RD, Tripathi P, et al. Moringa oleifera lam. (sahijan) - a plant with a plethora of diverse therapeutic benefits: an updated retrospection. Med Aromat Plants 2012;1: 1-8.

Peixoto JRO, Silva GC, Costa RA, et al. In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts. Asian Pac J Trop Med 2011: 201-204.

Rao PK, Rao DB, Kiran R, et al. In vitro antibacterial activity of Moringa Oleifera against dental plaque bacteria. J Pharm Res 2011;4: 695-697.

Suriaman E, Khasanah S. Screening for the antibacterial activity of moringa oleifera leaves, sea bidara leaves (strychnos ligustrina blume), and amoxicillin against staphylococcus aureus pathogenic bacteria. J Biota 2017;3: 21-26. (In Indonesia)

Meizarini A. The cytotoxicity of cyanoacrylate restorations in various ratio of powder and liquid using MTT assay. Maj Ked Gigi (Dent J) 2005;38: 20-24. (In Indonesia)

Khoswanto C. Cytotoxicity test of 50% citric acid conditioner dentin using MTT assay. Dent J 2008;41: 103-106.

Karim ANA, Ibrahim M, Kantayya SB, et al. Moringa oleifera Lam: targeting chemoprevention. Asian Pac J Cancer Prev 2016;17: 3675-3686.

Ambi AA, Abdurahman EM, Katsayal UA, et al. Toxicity evaluation of moringa oleifera leaves. Int J Pharmaceutic Res Inn 2011;4: 22-24.

Olayemi AT, Olanrewaju MJ, Oloruntoba AC. Toxicological evaluation of Moringa oleifera Lam seeds and leaves in wistar rats. Pharmacogn Comn 2016;6: 100-111.

Babychan N, Reshma JK, Mole SL et al. Analysis of antioxidant properties of moringa oleifera Lam in urban and coastal area. IJAR 2017;3: 1098-1101.

Stohs SJ, Hartman MJ. Review of the safety and efficacy of moringa oleifera phytother. Res 2015;29: 796-804.

Muhammad AA, Pauzi NAS, Arulselvan P, et al. In Vitro wound healing potential and identification of bioactive compounds from Moringa oleifera Lam. BioMed Res Int 2013: 1-11.


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