Original Article

Roselle flower petals extract inhibits periodontal pathogenic biofilms

James Sebastian, Armelia S. Widyarman

James Sebastian
Department of Microbiology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia

Armelia S. Widyarman
Department of Microbiology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia. Email: armeliasari@trisakti.ac.id
Online First: August 01, 2021 | Cite this Article
Sebastian, J., Widyarman, A. 2021. Roselle flower petals extract inhibits periodontal pathogenic biofilms. Journal of Dentomaxillofacial Science 6(2): 102-105. DOI:10.15562/jdmfs.v6i2.1122


Objectives: The present study aimed to determine the effect of roselle flower petals extract on Fusobacterium nucleatum and Porphyromonas gingivalis biofilms in vitro.

Material and Methods: Crystal violet biofilm assays were carried out to evaluate the effect of roselle flower petals extract on F. nucleatum ATCC-25586 and P. gingivalis ATCC-3327 biofilms. Each bacterium was cultured in Brain Heart Infusion broth for 48 h at 37°C under anaerobic conditions. Subsequently, 200 µL (107 CFU/mL) of bacterial suspension was distributed in a 96-well plate for 48 h to form both mono- and dual-species biofilms. Different concentrations of the roselle flower extract and 0.2% chlorhexidine-gluconate (positive control) were added to the biofilms in the wells and incubated for 1 h, 6 h, and 24 h.

Results: There was a significant biofilm reduction after treatment with roselle flower petals extract at all concentrations and incubation times compared to the negative control (p<0.05). The most effective concentrations for inhibiting the monospecies biofilms of F. nucleatum and P. gingivalis and the dual species biofilm were 100%, leading to a 99.8%, 100%, and 90.6% reduction of the biofilm compared to the control, respectively.

Conclusion: Roselle flower petals extract is effective at inhibiting F. nucleatum and P. gingivalis biofilms. This anti-biofilm agent may be developed as an alternative therapy to prevent periodontal disease. Future studies are needed to explore the mechanisms of action of the active compounds.

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