Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin


Maria Tanumiharja
Arfina S. Hamid
Aries C. Trilaksana
Christine A. Rovani
Nurhayati Natsir
Juni J. Nugroho
Indrya K. Mattulada
Lukman Muslimin


Objective: To examine the expression of caspase-3 and IL-1? following application of Pulp Out® on rabbit’s pulp teeth as well as histopathology.Material and Methods: The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation.Results: Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1? showed similarities to the expression of commercial devitalizing agent.Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.


How to Cite
Tanumiharja, M., Hamid, A. S., Trilaksana, A. C., Rovani, C. A., Natsir, N., Nugroho, J. J., Mattulada, I. K., & Muslimin, L. (2021). Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin. Journal of Dentomaxillofacial Science, 6(2), 119–123. https://doi.org/10.15562/jdmfs.v6i2.1232


Shalini S, Dorstyn L, Dawar S, et al. Old, new and emerging functions of caspases. Cell Death Differ 2015;22: 526-539.
2. Van-Opdenbosch N, Lamkanfi M. Caspases in cell death, inflammation, and disease. Immun 2019;50: 1352-1364.
3. Lossi L, Castagna C, Merighi A. Caspase-3 mediated cell death in the normal development of the mammalian cerebellum. Int J Mol Sci 2018;19: e3999.
4. Pradeep AR, Suke DK, Prasad MV, et al. Expression of key executioner of apoptosis caspase-3 in periodontal health and disease. J Investig Clin Dent 2016;7: 174-179.
5. Yang Y, Jiang G, Zhang P, et al. Programmed cell death and its role in inflammation. Military Med Res 2015;2: e12.
6. Rock KL, Kono H. The inflammatory response to cell death. Annu Rev Pathol 2008;3: 99-126.
7. Kaneko N, Kurata M, Yamamoto T, et al. The role of interleukin-1 in general pathology. Inflamm Regen 2019;39: e12.
8. Guan P-P, Guo J-W, Yu X, et al. The Role of cyclooxygenase-2, interleukin-1? and fibroblast growth factor-2 in the activation of matrix metalloproteinase-1 in sheared-chondrocytes and articular cartilage. Sci Rep 2015;5: e10412.
9. Lee C-H, Choi Y, Park S. Mandibular bone necrosis after use of paraformaldehyde-containing paste. Restor Dent Endod 2016;41: 332-337.
10. Tanumihadja M, Mattulada IK, Natsir N, et al. Potential combination of sidaguri root extract (Sida rhombifolia L.) and castor gum (Jatropha curcas L.) as a devitalizing agent. ODONTO 2019;6: 14-20. (In Indonesia)
11. Tang D, Kang R, Berghe TV, et al. The molecular machinery of regulated cell death. Cell Res 2019;29: 347-364.
12. Zille M, Ikhsan M, Jiang Y, et al. The impact of endothelial cell death in the brain and its role after stroke: A systematic review. Cell Stress 2019;3: 330-347.
13. Lugano R, Ramachandran M, Dimberg A. Tumor angiogenesis: causes, consequences, challenges and opportunities. Cell Mol Life Sci 2020;77: 1745-1770.
14. Honnegowda TM, Kumar P, Udupa EGP, et al. Role of angiogenesis and angiogenic factors in acute and chronic wound healing. Plast Aesthet Res 2015;2: 243-249.
15. Mantovani A, Dinarello CA, Molgora M, et al. Interleukin-1 and related cytokines in the regulation of inflammation and immunity. Immunity Rev 2019;50: 778-795.
16. Liu X, Guan N. Microglia and CNS interleukin-1 : beyond immunological concepts. Front, Neurol 2018;9: 8.
17. Mattulada IK. Histological observation of the dental pulp of M. nemestrina exposed to the sap of Jatropha curcas. J Dentomaxillofac Sci 2008;7: 19-25. (In Indonesia)
18. Asep S, Hening H, Gema SP, et al. Anticancer activity of jatrophone an isolated compound from Jatropha gossypifolia plant against hepatocellular cancer cell Hep G2 1886. Biomed Pharmacol J 2017;10: 667-673.
19. Westman J, Grinstein S, Marques PE. Phagocytosis of necrotic debris at sites of injury and inflammation. Front Immunol 2020;10: e3030.
20. Siregar F. Cytotoxicity of physic nut (Jatropha curcas L., Euphorbiaceae) latex by agar-overlay. J Kedokteran YASRI 2015;23: 143-148.
21. England H, Summersgill HR, Edye ME, et al. Release of interleukin-1? or interleukin-1? depends on mechanism of cell death. J Biol Chem 2014;289: 15942-15950.
22. Hangai S, Ao T, Kimura Y, et al . PGE2 induced in and released by dying cells functions as an inhibitory DAMP. Proc Natl Acad Sci USA 2016;113: 3844-3849.
23. Tanumihadja M, Mattulada IK, Natsir N, et al. Structural assessment of chemical constituent of sidaguri (Sida rhombifolia Linn.) and its ability to inhibit cyclooxygenase. Pesqui Bras Odontopediatria Clin Integr 2019;19: e4773.
24. Tanumihardja M, Natsir N, Mattulada IK, et al. Potent anti-inflammatory effect of root of sidaguri (Sida rhombifolia L) on rat periapical lesion model. Int J Toxi Pharm Res 2016;8: 412-415.

Most read articles by the same author(s)

> >>