Osteocalcin levels evaluation of freeze-dried homologus platelet-rich plasma in periodontal treatment

Abstract

Objective: The aim of this study was to evaluate enhanced osteocalcin levels by using freeze-dried homologous platelet-rich plasma (FD HPRP) in periodontal treatment to support the osteogenesis process. Material and Methods: FD HPRP was made by HPRP from the blood bank, then freeze-drying and for sterilization, the γ-radiation process was carried out with doses of 20 and 25 kGy. The osteoblast (Cell line MG63) was cultured to confluent and then treated with FD HPRP 20 kGy, FD HPRP 25 kGy. Osteocalcin levels were tested using enzyme-linked immunosorbent assay (ELISA) on the 7th and 14th days. The data were analyzed using a t test Results: There was a significant difference in the osteocalcin levels between FD HPRP 20 and FD HPRP 25 (p<0.05). Osteocalcin levels increased on day 14 in each group and the highest one on the 7th and 14th days was FD HPRP 25. Conclusion: The present results showed that FD HPRP with simple preparation could support the osteogenesis process