Irene E. Riuwpassa; St. Rafiah; I Wayan S.A

doi:10.15562/jdmfs.v7i1.192

Deteksi helicobacter pylori pada plak gigi dengan reverse transcriptionpolymerase chain reaction

Irene E. Riuwpassa ,

St. Rafiah ,

I Wayan S.A ,

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Abstract

Helicobacter pylori have been found associated with dental plaque. It was suggestedthat the oral environment may be one of the many potential pathways for theirtransmission. The aim of this study was to evaluate whether the oral cavity is apotential reservoir and possible sanctuary for Helicobacter pylori. Supragingival andsubgingival plaques were analyzed by a Helicobacter genus-specific reversetranscriptase-fragment was identified.

Keywords: H. pylori dental plaque RT-PCR

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References

Brooks GF, Butel SJ, Morse SA. Mikrobiologi kedokteran. Penerjemah dan Editor. Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Jakarta: Salemba Medika; 2001.
Guyton, Hall. Fisiologi kedokteran. Alih bahasa: Setiawan I, editor. Ed.9. Jakarta: EGC; 1997.
Price SA, Wilson LM. Patofisiologi. Alih bahasa: Brahm. Jakarta: EGC; 2005.
Lindhe J. Textbook of clinical periodontology. Copenhagen: Munksgaard; 1988.
Gebara ECE, Pannuti C, Faria CM. Prevalence of Helicobacter pylori detected by polymerase chain reaction in the oral cavity of periodontitis patients. Oral Microbiol Immunol 2004; 19 (4): 277-80.
Powledge TM. The polymerase chain reaction; 2005.
Mullis K. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 1986;
: 335-50.
Yuwono T. Teori dan aplikasi polymerase chain reaction. Yogyakarta: ANDI; 2006..
Ansubel F, Donald MC. Short protocols in molecular biology: the polymerase chain reaction, 3rd ed. St. Louis: John Wiley and Sons, Inc,; 1995.
Putra ST. Biologi molekuler kedokteran. Surabaya: Airlangga University Press; 1997.
Nguyen AMH, Engstrand L, Genta RM. Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. J Clin Microbiol 1992; 31: 783-7.
Umeda M, Kobayashi H, Hayashi J. High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients. J Periodontol 2003; 74: 129-34.
Departemen Kesehatan Republik Indonesia. Badan Penelitian dan Pengembangan Kesehatan.
Sjahrurachman A. Cara Genetis untuk Menentukan Kepekaan Bakteri Terhadap Antibiotika. Medika 2000; 26 (1)Januari.

How to Cite

Riuwpassa, I. E., Rafiah, S., & S.A, I. W. (2008). Deteksi helicobacter pylori pada plak gigi dengan reverse transcriptionpolymerase chain reaction. Journal of Dentomaxillofacial Science, 7(1), 38–46. https://doi.org/10.15562/jdmfs.v7i1.192

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[1] Brooks GF, Butel SJ, Morse SA. Mikrobiologi kedokteran. Penerjemah dan Editor. Bagian Mikrobiologi Fakultas Kedokteran Universitas Airlangga. Jakarta: Salemba Medika; 2001.

[2] Guyton, Hall. Fisiologi kedokteran. Alih bahasa: Setiawan I, editor. Ed.9. Jakarta: EGC; 1997.

[3] Price SA, Wilson LM. Patofisiologi. Alih bahasa: Brahm. Jakarta: EGC; 2005.

[4] Lindhe J. Textbook of clinical periodontology. Copenhagen: Munksgaard; 1988.

[5] Gebara ECE, Pannuti C, Faria CM. Prevalence of Helicobacter pylori detected by polymerase chain reaction in the oral cavity of periodontitis patients. Oral Microbiol Immunol 2004; 19 (4): 277-80.

[6] Powledge TM. The polymerase chain reaction; 2005.

[7] Mullis K. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 1986;

[8] : 335-50.

[9] Yuwono T. Teori dan aplikasi polymerase chain reaction. Yogyakarta: ANDI; 2006..

[10] Ansubel F, Donald MC. Short protocols in molecular biology: the polymerase chain reaction, 3rd ed. St. Louis: John Wiley and Sons, Inc,; 1995.

[11] Putra ST. Biologi molekuler kedokteran. Surabaya: Airlangga University Press; 1997.

[12] Nguyen AMH, Engstrand L, Genta RM. Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. J Clin Microbiol 1992; 31: 783-7.

[13] Umeda M, Kobayashi H, Hayashi J. High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients. J Periodontol 2003; 74: 129-34.

[14] Departemen Kesehatan Republik Indonesia. Badan Penelitian dan Pengembangan Kesehatan.

[15] Sjahrurachman A. Cara Genetis untuk Menentukan Kepekaan Bakteri Terhadap Antibiotika. Medika 2000; 26 (1)Januari.