Perbandingan sitotoksisitas Endomethasone, AH Plus, dan Apexit Plus terhadap sel fibroblas dengan teknik root dipping
The purpose of this study was to evaluate the lowest cytotoxicity of three endodonticsealers, Apexit Plus, AH Plus and Endomethasone that can be used safely. The crownof 30 intact lower premolars were removed at cementodentinal juntion and the teethwere prepared with crown down technique with # 20 initial file and # 30 masterapical file. After sterilization, the 30 teeth were randomly divided into three groupsand filled with gutta percha using laterally condensation technique and Apexit Plus,AH Plus, and Endomethasone as sealers. Apex of the roots was dipped 1.5 mm intoculture medium for 24 hours in incubator (root dipping technique). The mediumcontact was transformed into confluent chicken fibroblast. Cytotoxicity ofEndomethasone, AH Plus, and Apexit Plus were measured with hemocytometer assay.The results showed that percentage survived cells of Apexit Plus was 68%,Endomethasone 66.42%, and AH Plus 64%. The amount of exist cells were ApexitPlus>Endomethasone>AH Plus. There was no significant difference of cytotoxicityamong the sealers (P>0.05). The conclusion was the amount of exist cells were morethan 50% which means that the three root canal sealers were not toxic.
- Walton RE Torabinejad M. Principles and practice of endodontics. 3rd
- ed. Philadelphia WB Saunders; 2002. p. 12-3; 22-3; 240-1; 248-51.
- Happonen RP, Bergenholtz G. Text book of endodontology. Bergenholtz G, Horsted B, Reit, editor. Oxford: Blackwell; 2003. p. 261, 270-80.
- Ingle JI, Newton CW, West JD, Gutmann JL, Glicman GN, Korson BH, dkk. Obturation of the radicular space. In: Ingle JI, Bakland, editors. Endodontics, 5th ed. London: BC Decker Inc Hamilton; 2002. p. 579-96.
- Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin, zinc oxide-eugenol, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. J Int Endod 2002; 35:153-8.
- Leonardo RM, da Silva LAB, Filho MT, da Silva RS. Release of formaldehyde by 4 endodontic sealers. Oral Surg Oral Med Oral Pathol Radiol Endod 1999; 88: 221-5.
- Huang TH, Ding SJ, Kao CT, Lee ZD, Hsu TZ. Root canal sealers induce cytotoxicity and necrosis. J Mater Sci 2002; 15: 767-71.
- Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod 2000; 26: 228-9.
- Limkangwalmongkol S, Burtscher P, Abbott PV, Sandler AB, Bishop BM. A comparative study of the apical leakage of four root canal sealers and laterally condensed gutta-percha. J Endod 1991; 17(10): 495-9.
- Schrwarse T, Fiedler I, Leyhausen G, Geurtsen W. The cellular compatibility of five endodontic sealers during the setting period. J
- Endod 2002; 28: 784-6.
- Alberts B. Bray D, Lewis M, Raff M, Roberts K, Watson JD. Moleculer biology of the cell. 3rd Ed. New York: Garland Publishing; 1994. p. 1179.
- Briseno BM, Willershausen B. Root canal sealer cytotoxity with human gingival fibroblast. III. Calcium hydoxide-based sealers. J Endod 1992; 18: 110-3.
- Camps J, About I. Cytotoxicity testing of endodontic sealers: A new method. J Endod 2003; 29: 583-6.
- Sumawinata N. Evaluation of mutagenicity of three eugenol-containing material using Ames Test. Indonesian Dent J 2007; 14(1): 12-6.
- Suwarno. Kultur sel fibroblas embrio ayam sebagai feeder layer pertumbuhan sel hibridoma mencit dalam inkubator tanpa
- karbondioksida. Jurnal Ilmu Pengetahuan & Teknologi Universitas Airlangga 2003.
- Freshney RI. Culture of animal cells a manual 7778 of basic technique. 4th ed. New York: WileyLiss; 2000. p. 329-30; 1846.
- Sumawinata N. Survai perawatan endodonsia dalam praktik dokter gigi: Penelitian pendahuluan. J Epid Indonesia.2004; 3: 23-6.
- Pumarola J, Berastegui E, Brau E, Canalda C, Anta MTJ. Antimicrobial activity of seven root canal sealers. Oral Surg Oral Med Oral Pathol 1992; 74: 216-20.
- Beltes P, Koulaouzidou E, Kotoula V, Kortsaris AH. In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod Dent Traumatol 1995; 11: 245-9.
How to Cite
Rovani, C. A., Kamizar, K., & Usman, M. (2008). Perbandingan sitotoksisitas Endomethasone, AH Plus, dan Apexit Plus terhadap sel fibroblas dengan teknik root dipping. Journal of Dentomaxillofacial Science, 7(2), 69–77. https://doi.org/10.15562/jdmfs.v7i2.196