Original Article

Perbandingan sitotoksisitas Endomethasone, AH Plus, dan Apexit Plus terhadap sel fibroblas dengan teknik root dipping

Christine A. Rovani , Kamizar Kamizar, Munyati Usman

Christine A. Rovani
Bagian Ilmu Konservasi Gigi Fakultas Kedokteran Gigi Universitas Indonesia Jakarta, Indonesia. Email: ch_rovani@yahoo.com

Kamizar Kamizar
Bagian Ilmu Konservasi Gigi Fakultas Kedokteran Gigi Universitas Indonesia Jakarta, Indonesia

Munyati Usman
Bagian Ilmu Konservasi Gigi Fakultas Kedokteran Gigi Universitas Indonesia Jakarta, Indonesia
Online First: October 30, 2008 | Cite this Article
Rovani, C., Kamizar, K., Usman, M. 2008. Perbandingan sitotoksisitas Endomethasone, AH Plus, dan Apexit Plus terhadap sel fibroblas dengan teknik root dipping. Journal of Dentomaxillofacial Science 7(2): 69-77.


The purpose of this study was to evaluate the lowest cytotoxicity of three endodontic
sealers, Apexit Plus, AH Plus and Endomethasone that can be used safely. The crown
of 30 intact lower premolars were removed at cementodentinal juntion and the teeth
were prepared with crown down technique with # 20 initial file and # 30 master
apical file. After sterilization, the 30 teeth were randomly divided into three groups
and filled with gutta percha using laterally condensation technique and Apexit Plus,
AH Plus, and Endomethasone as sealers. Apex of the roots was dipped 1.5 mm into
culture medium for 24 hours in incubator (root dipping technique). The medium
contact was transformed into confluent chicken fibroblast. Cytotoxicity of
Endomethasone, AH Plus, and Apexit Plus were measured with hemocytometer assay.
The results showed that percentage survived cells of Apexit Plus was 68%,
Endomethasone 66.42%, and AH Plus 64%. The amount of exist cells were Apexit
Plus>Endomethasone>AH Plus. There was no significant difference of cytotoxicity
among the sealers (P>0.05). The conclusion was the amount of exist cells were more
than 50% which means that the three root canal sealers were not toxic.

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