A cytotoxic evaluation of 7 th generation dentin bonding agent on human pulp cells

Abstract

Dentin bonding agents are resin based materials that used in clinical dentistry inorder to prevent leakage and promote adherent of filling material to the enamel anddentin. However, the polymerization of dentin bonding agents will release residualmonomer that may interact with pulp tissue. This in vitro study is aimed to evaluatethe cytotoxicity of new 7thgeneration dentin bonding agents (G Bond) on stem cellsfrom human exfoliated deciduous teeth (SHEDs). The proliferated human pulp cellswere incubated at 37ï‚° C for 48 hours. Under aseptic conditions, extracted testspecimen were plated in 96 well of tissue tray. SHEDs were placed on each well withdifferent concentration of dentin bonding agents, and then incubated at 37ï‚° C for 72hours exposure. The cytotoxic effect was recorded by using MTT assay method.Statistical analysis showed that higher concentration of dentin bonding agents (IC=0.035 mg/ml)exerts higher toxic effect to the human pulp cells (SHEDs). This studyconcluded that pulpal reaction to dentin bonding agent may depend on number ofapplication procedure.