A cytotoxic evaluation of 7 th generation dentin bonding agent on human pulp cells

Dentin bonding agents are resin based materials that used in clinical dentistry in
order to prevent leakage and promote adherent of filling material to the enamel and
dentin. However, the polymerization of dentin bonding agents will release residual
monomer that may interact with pulp tissue. This in vitro study is aimed to evaluate
the cytotoxicity of new 7
th
generation dentin bonding agents (G Bond) on stem cells
from human exfoliated deciduous teeth (SHEDs). The proliferated human pulp cells
were incubated at 37 C for 48 hours. Under aseptic conditions, extracted test
specimen were plated in 96 well of tissue tray. SHEDs were placed on each well with
different concentration of dentin bonding agents, and then incubated at 37 C for 72
hours exposure. The cytotoxic effect was recorded by using MTT assay method.
Statistical analysis showed that higher concentration of dentin bonding agents (IC
=
0.035 mg/ml)exerts higher toxic effect to the human pulp cells (SHEDs). This study
concluded that pulpal reaction to dentin bonding agent may depend on number of
application procedure.