Original Article

Adanya korelasi kadar TNF-α antara pemeriksaan hapusan lesi dengan pemeriksaan darah perifer pasien stomatitis aftosa rekuren Correlation of TNF-α between lesional swab and peripheral blood of mononuclear cell of recurrent aphthous stomatitis patient

Ali Yusran , Erni Marlina, Sumintarti Sumintarti

Ali Yusran
Bagian Oral Medicine, Fakultas Kedokteran Gigi Universitas Hasanuddin Makassar, Indonesia. Email: e.marlina@yahoo.co.id

Erni Marlina
Bagian Oral Medicine, Fakultas Kedokteran Gigi Universitas Hasanuddin Makassar, Indonesia

Sumintarti Sumintarti
Bagian Oral Medicine, Fakultas Kedokteran Gigi Universitas Hasanuddin Makassar, Indonesia
Online First: June 30, 2011 | Cite this Article
Yusran, A., Marlina, E., Sumintarti, S. 2011. Adanya korelasi kadar TNF-α antara pemeriksaan hapusan lesi dengan pemeriksaan darah perifer pasien stomatitis aftosa rekuren Correlation of TNF-α between lesional swab and peripheral blood of mononuclear cell of recurrent aphthous stomatitis patient. Journal of Dentomaxillofacial Science 10(2): 71-75.


Recurrent aphthous stomatitis (RAS)is a common oral mucosal disease in human. This disease affect 20-25% of
population. The etiology remains unclear, but some of cytokine such as TNF-α is suspected to contribute in the
pathogenesis of RAS. TNF-α is commonly measured by PBMC and lesional biopsy. These are invasive method and
high skilled requirement. Another study used lesional swab to measure this cytokine without correlating the result
with TNF-α measured by PBMC level. This study aimed to asses TNF-α level correlation between lesional swab and
PBM of RAS patients. The study involved 10 subjects by inclusion criteria. Subjects have lesion swab and PBMC
examined. Samples were analyzed by ELISA method. There is a correlation between lesional swab and PBMC of
TNF-α level RAS patients but this correlation did not statistically significant.

Keywords


References

Field A, Longman L. Tyldesley’s oral medicine. 5 Ed. Oxford: Oxford University Press; 2003.p.3-9.

Greenberg SM. Ulserative, vesicular and bullous lesions. In: Greenberg MS, Glick M, editors. Burket’s oral medicine diagnosis and treatment. 10 Ed. Hamilton: BC Decker, Inc; 2003. p.50-102.

Natah SS, Konttinen YT, Enattah NS, Ashammaktil N, Sharkey KA, Hayrinen-Immonen R. Recurrent aphthous ulcers today : a review of the growing knowledge. Int J Oral Maxillofac Surg 2004; 33:221-34.

Scully C. The diagnosis and management of reccurent aphthous stomatitis. J Am Dent Assoc 2003; 134: 200-7.

Buno IJ, Huff JC, Weston WL, Cook DT, Brice SL. Elevated levels of interferon gamma, tumor necrosis alpha, interleukin 2, 4 and 5, but not interleukin 10, are present in recurrent aphthous stomatitis. Arch Dermatol 1998; 134: 827-31.

Lin SS, Chou YM, Ho CC. Study of the viral infection and cytokines associated with recurrent aphthous ulceration. Microbes and Infection 2005;7: 635-44.

Yamamoto T, Yoneda K, Ueta E, Osaki T. Serum cytokines, interleukin-2 receptors, and soluble intercellular adhesion molecule-1 in oral disorders, Oral Surg Oral Med Oral Pathol Oral Radiol

Endodon 1994; 78: 737-5.thth Ali Yusran dkk: Korelasi kadar TNF-α antara pemeriksaan hapusan lesi

Natah SS. Recurrent aphthous ulceration: imunopathological aspects. [thesis]. Helsinki: University of Helsinki; 2001.p.30-60.

Natah SS, Immonen HR, Hietanen J, Patinen P, Malmstrom M, Savilahti E, et al. Increased density of lymphocytes bearing γ/ T-ce;p; receptors in recurrent aphthous ulceration (RAU). Int J Oral Maxillofac Surg 2000; 29: 375-80.

Gerber S, Bongiovanni AM, Ledger WJ, Witkin SS. Interleukin-1β gene polymorphism in women with vulvar vestibulitis syndrome. Eur J Obst Gyn Repro Biol 2003; 107: 74-7.

Guimaraes ALS, Correia-Silva JF, de Santos RA, Victoria JMN, Diniz MG, Costa FD, Gomes RS. Investigation of functional gene polymorphisms IL1β, IL-6, IL-10, and TNF-α in individual with

recurrent aphthous stomatitis. Arch Oral Biol 2007;52: 268-72.

Marlina E. Teknik hapusan mukosa mulut ada ulser RAS minor melalui perbedaan kadar TNF-α dan IL10. [thesis]. Surabaya: Univ. Airlangga; 2010.

Lehner T. Pathology of recurrent oral ulceration and oral ulceration in Behçet’s syndrome: light, electron and fluorescence microscopy. J Pathol 2010; 97:481-93.

Mills MP, Mackler BF, Nelms DC, Peavy DL. Quantitative distribution of inflammatory cells in recurrent aphthous stomatitis. J Dent Res 2010; 59:562-6.

Schroeder HE, Müller-Glauser W, Sallay K. Stereologic analysis of leukocyte infiltration in oral ulcers of developing Mikulicz aphthae. Oral Surg Oral Med Oral Pathol 2010; 56: 629-40.

Hayrinen-Immonen R. Immune-activation in recurrent oral ulcer ROU. Scand J Dent Res 2010;100: 222-7.

Pinchuk G. Theory and problems of immunology. New York: McGraw-Hill; 2002. p.158-81.

Roitt IM, Delves PJ. Roitt’s essential immunology.10th Ed. Massachusetts: Blackwell Pub.; 2008. p.177-89.

Lewkowicz N, Lewkowicz P, Banasik M,Kurnatowska A, Tchorzewski. Predominance of Type 1 cytokines and decreased number of

CD4+CD25+high T regulatory cells in peripheral blood of patients with recurrent aphthous ulcerations. Immunol Letters 2005; 99: 57-62.

Hill JA. Cytokines in human reproduction. Toronto: Wiley-Liss; 2000. p.80-1.

O’Gorman RGM, Donnenberg DA., eds. Handbook of human immunology. 2nd Ed. London: CRC Press; 2008. p.495-525.

Spafford MF, Koch WM, Reed AL, Califano JA,Xu LH, Eisenberger CF, Yip L, et al. Detection of head and neck squamous cell carcinoma among exfoliated oral mucosal cells by microsatellite analysis.Pubmed 2008 [cited 2008 Nov 15] available from URL:www.ncbi.hlm.nih.gov/pubmed.

Dragnev KH, Petty JW, Shah S, Biddle a, Desai NB, Memoli V. Bexarotene and erlotinib for arodigestive tract cancer. J Clin Oncol 2005; 23:8757-64.

Pate SM, Hedge SP, Sibley DA, Russel MW, Hook III EW, MesteckyJ. urethral cytokine and immune responses in Chlamydia trachomatis-infected males. Infection and Immunity 2001; 11: 7178-81.

Agnew KJ, Aura J, Nunez N, Lee Z, Lawler R, Richardson CE, et al. Effect of semen on vaginal fluid cytokines and secretory leukocyte protease inhibitor. Inf Dis Obs Gyn 2008; 1-4.

Faro CJ, Holler LM, Bishop K. Comparison of vaginal cytokine collection methods. Am J Reprod Immunol 2006; 55(5): 315-20.

Savage WN, Boras VV. Recurrent aphthous ulcerative disease: presentation and management. Aus Dent J 2007; 52(1): 10-5


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