Original Article

Viabilitas neutrofil yangdiinkubasi dalamekstrakrimpangtemulawak(Curcuma xanthorrhiza ) dan dipapar dengan Streptococcus mutans (Viability of neutrophil incubated in temulawak rhizome extract (Curcuma xanthorrhiza) and exposed by Streptococcus mutans)

Ika Wahyu Purnamasari , Pudji Astuti, Tantin Ermawati

Ika Wahyu Purnamasari
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember Jember, Indonesia. Email: iwcassie@gmail.com

Pudji Astuti
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember Jember, Indonesia

Tantin Ermawati
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember Jember, Indonesia
Online First: October 30, 2014 | Cite this Article
Purnamasari, I., Astuti, P., Ermawati, T. 2014. Viabilitas neutrofil yangdiinkubasi dalamekstrakrimpangtemulawak(Curcuma xanthorrhiza ) dan dipapar dengan Streptococcus mutans (Viability of neutrophil incubated in temulawak rhizome extract (Curcuma xanthorrhiza) and exposed by Streptococcus mutans). Journal of Dentomaxillofacial Science 13(3): 135-140. DOI:10.15562/jdmfs.v13i3.403


Streptococcus mutans are bacteria that have important role in caries development. When caries lesion is developing, it
may invade to deeper tissues such as dental pulp and initiate cellular defenses such as neutrophil. Neutrophil is type of
leukocytes which are important in phagocytosis process. If neutrophil fails in phagocytosis, neutrophil will be lysis and
cause viability of neutrophil unprotected. Temulawak rhizome extract is predicted to increase viability of neutrophil
because it contains curcuminoids, volatile oil, saponin, flavonoid, and tannin. This research aimed of this research was
to determine the viability of neutrophil exposed by S.mutans and incubated in temulawak rhizomes extract 2.5%, 5%,
7.5%,10%, and 20%. Twenty four samples were divided into 6 groups, consisting of negative control, temulawak rhizome
extract 2.5%, 5%, 7.5%, 10%, and 20%. The viability of neutrophil was observed under an inverted microscope using
trypan blue staining. This study concluded that temulawak rhizome extract 2.5%, 5%, 7.5%, and 10% could increase
viability of neutrophil exposed by S.mutans. Temulawak rhizome extract 5% has the effective concentration to increase
viability of neutrophil.

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