Original Article

Biji kopi meningkatkan ekspresi glutathione padamonosit secara in vitro (Coffee beans increase glutathione expression to monocyes in vitro)

Roedy Budirahardjo

Roedy Budirahardjo
Bagian Pedodontik Fakultas Kedokteran Gigi Universitas Jember Jember, Indonesia. Email: roedibudirahardjo@yahoo.com
Online First: February 28, 2015 | Cite this Article
Budirahardjo, R. 2015. Biji kopi meningkatkan ekspresi glutathione padamonosit secara in vitro (Coffee beans increase glutathione expression to monocyes in vitro). Journal of Dentomaxillofacial Science 14(1): 61-64. DOI:10.15562/jdmfs.v14i1.428


Some research stated that coffee may act as an antioxidant, but has not mentioned concentrations can distinguish the
production of antioxidants. One of the important antioxidant roles in preventing various diseases is GSH. The research
is intended to analyze the expression of antioxidant GSH of coffee beans in vitro. Furthermore, the culture of
peripheral blood monocytes of healthy people as much as 6 cc, mixed with the anticoagulant heparin. Ficoll-Hypaque
centrifugation and suspended in RPMI 1640 medium Buffy coat separated, washed, resuspended. Cells were placed
on plate 96-well microtiter of 8 x 105 cells/well, and incubated. Then added RPMI, incubated and lymphocytes
discarded. After washing, complete medium was added and incubation. Monocytes reacted with coffee with various
concentrations (4 groups) and incubated 18 hours. GSH then analyzed by immunocytochemistry method. GSH cells
expressing brown and counted under a light microscope (400x). The results indicated that a concentration of 3 g coffee
brewed in 300 mL of hot water has the greatest ability to increase GSH. It was concluded that coffee can improve
GSHexpression on monocytes were exposed to DMBA, and the higher the concentration of the coffee bean, the higher
the expression of GSH.

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