Original Article

The effect of ethanol extract of myrmecodia pendens to TGF-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya)

Efa Ismardianita , Ellyza Nasrul, Yanwirasti Yanwirasti, Mieke Hemiawati

Efa Ismardianita
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University Baiturrahmah, Padang, Indonesia. Email: efa_ismardianita@yahoo.co.id

Ellyza Nasrul
Department of Clinical Pathologic, Faculty of Medicine, Andalas University, Padang, Indonesia

Yanwirasti Yanwirasti
Departement of Biomedic, Faculty of Medicine, Andalas University, Padang, Indonesia

Mieke Hemiawati
Departement of Microbiology, Padjajaran University, Bandung, Indonesia
Online First: December 01, 2017 | Cite this Article
Ismardianita, E., Nasrul, E., Yanwirasti, Y., Hemiawati, M. 2017. The effect of ethanol extract of myrmecodia pendens to TGF-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya). Journal of Dentomaxillofacial Science 2(3): 150-154. DOI:10.15562/jdmfs.v2i3.647


Objective: Myrmecodia pendens is often used as a traditional medicine to treat various diseases. A previous study to ethanol extract of sarang semut plant (Hypnophytum furmicarum jack) 4.65 mg was proven effective in fastening establishment of socket granulation tissue after tooth extraction, the Myrmecodia pendens 3% also fasten the healing process of soft tissue wound after tooth extraction. The aim of this study is to determine the effect of ethanol extract of 10%  Myrmecodia pendens to the expression of TGF-β1, and the number of osteoblasts after tooth extraction.

Material and Methods: this study used 24 male marmoots divided into 2 groups. The entire groups had been extracted in the 1st left incisor mandibula, into the socket control group applied 0,1 ml of 0.5% CMC. The treatment group was given 0,1 ml ethanol extract of Myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3, 7, 14 and 21, marmoots were decapitated and histopathologic preparations then observed the expression of TGF-β1, and the number of osteoblast cell. Data was analyzed by Kruskall-wallis test with probability p<0.05.

Results: Based on the Kruskal-wallis test, there was a significant difference of the TGF-β1 expression and the number of osteoblast cell which had giving ethanol extract of Myrmecodia pendens between the control group and group treatment (p<0.05).

Conclusion: the ethanol extract of Myrmecodia pendans can help the wound healing process after tooth extraction by increasing the expression of  TGF-β1, and osteoblast cells number.

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