Original Article

The correlation of consuming thymoquinone extract of nigella sativa to tooth socket re-epithelization on diabetic-induced rats

Mei Syafriadi , Putri R. Yusuf, Sabrina M. Pratama, Dina Z. Ummah, Khoirul Amalia

Mei Syafriadi
Department of Biomedical Science, Laboratorium of Oral Pathology, Faculty of Dentistry, Jember University, Jember, Indonesia. Email: didiriadihsb@gmail.com

Putri R. Yusuf
Department of Biomedical Science, Laboratorium of Oral Pathology, Faculty of Dentistry, Jember University, Jember, Indonesia

Sabrina M. Pratama
Department of Biomedical Science, Laboratorium of Oral Pathology, Faculty of Dentistry, Jember University, Jember, Indonesia

Dina Z. Ummah
Department of Biomedical Science, Laboratorium of Oral Pathology, Faculty of Dentistry, Jember University, Jember, Indonesia

Khoirul Amalia
Department of Biomedical Science, Laboratorium of Oral Pathology, Faculty of Dentistry, Jember University, Jember, Indonesia
Online First: August 01, 2018 | Cite this Article
Syafriadi, M., Yusuf, P., Pratama, S., Ummah, D., Amalia, K. 2018. The correlation of consuming thymoquinone extract of nigella sativa to tooth socket re-epithelization on diabetic-induced rats. Journal of Dentomaxillofacial Science 3(2): 79-83. DOI:10.15562/jdmfs.v3i2.742


Objective: The purpose of this research is to determine the effect of Tq to re-epithelialization at the margin of tooth socket post-extraction in diabetic rats.

Material and Methods: Used 27 rats were induced diabetic with streptozotocin (STZ) dose of 50 mg/kg body weight of rats which divided into 3 groups that has been stained with hematoxillin-eosin and cytokeratin 19. They are P1 treated with thymoquinone (Tq), P2 treated with metformin, and K without treatment. Each group consist of 9 rats were divided into 3 subgroups based on day of observation. They are 3rd, 7th and 10th day post-extraction. Re-epithelialization was observed at the margin of the tooth socket that has been stained with hematoxillin-eosin and cytokeratin 19

Results: showed there was an improvement of re epithelialization percentage in the Tq treatment group compared with P2 and K groups.

Conclusion: Thymoquinone extract may decrease blood glucose level and increasing re-epithelialization in margin of the tooth socket after extraction in diabetic rats.

References

Wijaya R, Wu L. Inhibition of a-glukosidase and amylase

activities of some Indonesian Herbs: In Vitro Study. The

th ASEAN food Conference. 2011

Guyton AC, Hall JE. Buku Ajar Fisiologi Kedokteran. 11th

ed. Jakarta: EGC; 2006.

Kementerian Kesehatan. Riset Kesehatan Dasar. 2013.

Sudoyo AW, Setiyohadi B, Alwi I, et al. Buku Ajar Ilmu

Penyakit Dalam. Jakarta: Internal Publishing; 2009.

Al-Farabi MJ. Antibodi terhadap advanced glycation end

product, cara mutakhir pencegahan komplikasi diabetes

mellitus. CDK 2013;40: 807-814.

Collison KS, Parhar RS, Saleh SS, et al. RAGE- ediated neutrophil

dysfunction is evoked by advanced glycation end

products (AGEs). J Leukoc Biol 2002;71: 433-444.

Abiko Y, Selimovic D. The mechanism of protracted

wound healing on oral mucose in diabetes. Review Bosn J

Basic Med Sci 2010;10: 186-191.

Radoi V, Lixandru D, Mohora M, et al. Advanced glycation

end products in diabetes mellitus: mechanism of action and

focused treatment. Proc Rom Acad Series B 2012;1: 9-19.

Huang S, Dang H, Huynh W, et al. The healing of dental

extraction sockets in patients with type 2 diabetes on

oral hypoglycaemics: a prospective cohort. Aust Dent J

;58: 89-93.

Torres-Lagares D, Srrera-Figallo MA, RomeroRu’iz MM,

et al. Update on dry socket: a review of the literature. Med

Oral Patol Oral Cir Bucal 2005;10: 77-85.

Vernillo AT. Dental consideration for the treatment of

patients with diabetes mellitus. Am Dent Asscoc 2003;134:

-33.

Davidson J, Howlett H. New prolonged-release metformin

improves gastrointestinal tolerability. Br J Diabetes Vasc

Dis 2004;4: 273-277.

Aksay E, Yanturali S, Bayram B, et al. A rare side effect of

metformin: metformin induced hepatoxicity. Turk J Med

Sci 2007;37: 173-175.

Kaleem M, Kirmani D, Asif M, et al. Biochemical effect

of nigella sativa l seeds in diabetic rats. Indian J Exp Biol

;44: 745-748.

Paarakh PM. Nigella sativa linn. a comprehensive review.

Indian J Nat Prod Resour 2010;1: 409-429.

Safferling K, Sutterlin T, Westpal K, et al. Wound

healing revised: a novel re-epithelialization mechanism

revealed by in vitro and in silico models. J Biophys

Biochem Cytol 2013;203: 691-709.

Pari, Sankaranarayanan C. Beneficial effects of thymoquinone

on hepatic key enzymes in streptozotocinnicotinamide

induced diabetic rats. Life Sciences 2009;85:

-834.

Ramirez H, Patel SB, Pastar I. The role of TGF-β signaling

in wound epithelization. WOUND CARE 2014;3: 482-491.

Abdelmeguid NE, Fakhoury R, Kamal SM, et al. Original

article: effects of nigella sativa and thymoquinone on

biochemical and subcellular changes in pancreatic BCells

of streptozotocin induced diabetic rats. J Diabetes

;2: 256-266.

Yuan L, Ziegler R, Hamann A. Inhibition of phosphoenol

pyruvate carboxykinase gene experession by metformin in

cultured hepatocytes. Chin Med J 2002;115: 1843-1848.

Graham GG, Punt J, Arora M, et al. Clinical pharmacokinetics

of metformin. Clin Pharmacokinet 2011;50: 81-98.

Gloire G, Legrand-Poels S, Piette J. NF-kB activation

by reactive oxygen species: fifteen years later. Biochem

Pharmacol 2006;72: 1493-1505.

Koivisto L, Hӓkkinen L, Larjava H. Reepithelization of

wound healing: cell biology and clinical management 1st ed.

John Wiley & Sons Inc. 2012.


No Supplementary Material available for this article.
Article Views      : 92
PDF Downloads : 66