Original Article

Antibacterial activity of essential oil extracts from Curcuma xanthorrhiza roxb. rhizomes against bacteria causing pulp necrosis

Dwi Prijatmoko , Nakhita L. Syafira, Pujiana E. Lestari

Dwi Prijatmoko
Department of Biomedical Science, Faculty of Dentistry, Jember University, Jember, Indonesia. Email: gawat_76.fkg@unej.ac.id

Nakhita L. Syafira
Department of Biomedical Science, Faculty of Dentistry, Jember University, Jember, Indonesia

Pujiana E. Lestari
Department of Biomedical Science, Faculty of Dentistry, Jember University, Jember, Indonesia
Online First: December 01, 2018 | Cite this Article
Prijatmoko, D., Syafira, N., Lestari, P. 2018. Antibacterial activity of essential oil extracts from Curcuma xanthorrhiza roxb. rhizomes against bacteria causing pulp necrosis. Journal of Dentomaxillofacial Science 3(3): 144-148. DOI:10.15562/jdmfs.v3i3.763


Objective: To analyze antibacterial activity of essential oil extracted from Curcuma xanthorrhiza Roxb. rhizomes against bacteria causing pulp necrosis, Enterococcus faecalis, and Fusobacterium nucleatum.

Material and Methods: The method used in this research was disc-diffusion on MH-A media, based on the standard protocol of National Committee for Clinical Laboratory Standards (NCCLS). This method used 4 replications in each of the study group. The study group consists of 4 different concentration groups (25%, 50%, 75% and 100% ) of Temulawak rhizomes essential oil extracts, ChKM and Cresophene were used as the positive control group and DMSO (10%) and Tween 80 (0.5%) was used as the negative control group.

Results: The inhibition zones of 25%, 50%, 75%, and 100% of temulawak rhizomes essential oil extracts against E. faecalis were 8.17 mm, 8.54 mm, 9.53 mm, and 10.17 mm, respectively. Whereas, the inhibition zones of 25%, 50%, 75%, and 100% of temulawak rhizomes essential oil extracts against F. nucleatum were 8.54 mm, 8.95 mm, 9.65 mm and 10.75 mm, respectively. Data analysis using Kruskal-Wallis in both of bacteria showed p=0.00 (p<0.05) and therefore, significant levels of inhibition.

Conclusion: Essential oil extracts from C. xanthorrhiza Roxb. rhizomes has medium antibacterial activity against E. faecalis and F. nucleatum.

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