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Steeping from green and black robusta coffee beans increase viability of Peripheral Blood Mononuclear Cells (PBMC) and salivary leukocytes which is induced by streptococcus mutans



Abstract

Objective: Analyzing viability of PBMC and salivary leukocytes  by steeping green coffee and black coffee seeds from Robusta coffee after induced with S. mutans.Material and Methods: Blood and saliva of healthy people added with histopaque centrifugation and suspended in RPMI 1640. The cells were placed in 24-well microtiter plate, treated according to group. The research groups: control: induced by S. mutans, black coffee (2.5%, 5%, 10%), Green coffee (2.5%, 5%, 10%). All coffee groups, both PBMC and saliva groups were induced by S. mutans. Viability was stainted with Tripan Blue. Cells were counted under an inverted microscope with magnification 400 times from an average of 5 fields of view. The data obtained were ANOVA followed by LSD test.Results. ANOVA and LSD analysis showed significant differences (0.01<p). The higher of concentration of Robusta coffee, the more cells that live. On the other hand, Green coffee increased the viability of PBMC and salivary leucocytes more than black coffee. Suspected, because antioxidant of the green coffee that more higher than black coffee, so it is better able to protect cells.Conclusion. Steeping Green and black coffee increase viability of PBMC and leucocytes which is induced by S. mutans.
Keywords: Blackcoffee Green coffee Leucocytes PBMC Viability
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References

  1. References
  2. Editorial Health Secret. Efficacy of Bombastic coffee. Jakarta: PT Elex Media Komputindo; 2010. p. 4-6.
  3. Coralie J. Dupas, Agnès C. Marsset-Baglieri, Claire S. Ordonaud, Fabrice MG Ducept, Marie-Noëlle Maillard. Coffee Antioxidant Properties: Effects of Milk Addition and Processing Conditions. Issue of the Journal of Food Science 2006 April;Vol 71, Issue 3: S253-S258.
  4. Natella F, Nardini M, Belelli F, Pignatelli M, Di Santo S, Ghiselli A, Violi F, Scaccini C. Effect of coffee drinking on platelets: inhibition of aggregation and incorporation phenols. Br J Nutr 2008 Dec;100 (6): 1276-82.
  5. Scalbert Augustin and Gary Williamson. Dietary Intake and Bioavailability of Polyphenols. J. Nutr 2000August 1; vol. 130 no. 8: 2073S-2085S.
  6. Mulato Sri, Edy Suharyanto. Coffee, steeping, and Health, Coffee and Cocoa Research Center Indonesia. Jember; 2010. p. 27-44.
  7. Magdalena Kwolek-Mirek Renata Zadrag-Tecza. Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Research 2014 November 1;Vol. 14 Issue 7: 1068–1079,https://doi.org/10.1111/1567-1364.12202
  8. Arun Kumar. Why Study Cell Viability, Cell Proliferation and Cytotoxicity?, 2018 March. http://www.enzolifesciences.com/science-center/, accessed July 3, 2018.
  9. PC Anila Namboodiripad and Sumathi Kori. Can coffee prevent caries?. J Conserv Dent 2009 Jan-Mar; 12(1): 17–21.
  10. Namboodiripad P., K. Srividya: Can Coffee Prevent Caries? - An In-Vitro Study. The Internet Journal of Dental Science 2009; 7(2). DOI: 10.5580/101c.
  11. Lestari Pujiana Endah, I Dewa Ayu Ratna Dewanti, Roedy Budirahardjo. Robusta Coffee Beans Increase Levels of TNF-α as A Response to Streptoccus mutans. 1st International Conference on Medicine and Health Sciences, 2016 Aug 31 st – Sep 1st. p. 162-168.
  12. Dewanti I Dewa Ayu Ratna Dewanti. Robusta Coffee Beans Decrease of Inflammation in Dental Caries, 1st International Conference on Medicine and Health Sciences. 2016 Aug 31 st – Sep 1st. pp. 173-176.
  13. Akhlaghi M, Bandy B,. Review article: mechanisms of flavonoid protection against myocardial ischemia– reperfusion injury. Journal Molecullar and Cellular Cardiology 2009;46. p. 309–317.
  14. Badria AF, El-Belbasi HI, Sobh MM, Badria FA,. Parallelism study between biochemical, immunological and histochemical parameters of liver injury induced by carbon tetrachloride on rats. Journal American Science 2011;7(5). p. 581-591
  15. Halliwell B, Gutteridge JMC,. Free Radicals In Biology And Medicine. Ed ke-4. Oxford, UK: Oxford University Press. Hanani E, Mun’im A, Sekarini R. 2005. Identifikasi senyawa antioksidan dalam spons Callyspongia sp. dari Kepulauan Seribu. Majalah Ilmu Kefarmasian 2007;2(3): 127-133.
  16. Lotito SB, Fraga CG,. Catechins delay lipid oxidation and alpha-tocopherol and beta-carotene depletion following ascorbate depletion in human plasma. Proceeding of The Society for Experimental Biology and Medicine 2000;225. p. 32–38.
  17. Middleton E Jr, Kandaswami C, Theoharides TC,. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacology Review 2000;52. p. 673–751
  18. Hecinovic, I., Cvitanovic, A>B., Horzic, D., & Komes. Comparation Study of Polyphenols and Caffein in Different Coffee Varietas Affected by The Degree of Roasting. Foof Chemistry 2011;129(3). p. 91-1000.
  19. Castelnovo, A.D., Giuseppe, R.D., Lacoviello, L., & Gaetano, G.D. Consumption of Cocoa, Tea and Coffee and Risk Cardiovascular Disease. European Journal of Internal Medicine 2012; 23(1). p.15-25.
  20. Jaehak Lee, Kandhasamy Sowndhararajan, Mihae Kim, Jaehun Kim, Daeho Kim, Sunpyo Kim, Gur-Yoo Kim, Songmun Kim, Jin-Woo Jhoo. Antioxidant, inhibition of α-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia argutastem. Saudi Journal of Biological Sciences 2014 December; 21(6). p. 532-538.
  21. Wen-Fei Chiou, Chieh-Fu Chen, and Jin-Jung Lin. Mechanisms of suppression of inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells by andrographolide. Br J Pharmacol 2000 Apr; 129(8). p. 1553–1560.
  22. Soohan Jung, Min Hyung Kim, Jae Hee Park, Yoonhwa Jeong, and Kwang Suk Ko. Cellular Antioxidant and Anti-Inflammatory Effects of Coffee Extracts with Different Roasting Levels. Journal of Medicinal Food. Published Online 1 Jun 2017;20(6). https://doi.org/10.1089/jmf.2017.3935
  23. Wen-Jye Yen, Bor-Sen Wang, Lee-Wen Chang, and Pin-Der Duh. Antioxidant Properties of Roasted Coffee Residues. J. Agric. Food Chem 2005 March 11; 53(7). p. 2658–2663.
  24. Gayathri Gunalan, Neelima Myla and R. Balabhaskar. In vitro Antioxidant Analysis of Selected Coffee Bean Varieties. Journal of Chemical and Pharmaceutical Research 2012; 4(4). p. 2126-2132.
  25. Gunawijaya & Arhana. The Role of Nitrogen Oxides in Infection. Denpasar: Sari Pediatri. 2000 August;2(2). p. 113 – 119.
  26. Ji Hee Jeong, Hee Rok Jeong,Yu Na Jo, Hyun Ju Kim, Uk Lee, and Ho Jin Heo. Antioxidant and Neuronal Cell Protective Effects of Columbia Arabica Coffee with Different Roasting Conditions. Prev Nutr Food Sci 2013 Mar;18(1). p. 30–37.
  27. Blanco J & Garcia M. Immune response to fungal infections. Veterinary Immunology and Immunopathology. Spain : Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid 2008. p. 47-70.
  28. Regina CLA, Ana PLV, SimoneF, et al. Phytochemical composition, antioxidant activity, and the effect of the aqueous extract of coffee (coffea arabica L.) bean residual press
  29. cake on the skin wound healing. Oxid Med Cell Longev
  30. ;2016: 1923754.

How to Cite

Dewanti, I.-D. A., Budirahardjo, R., Yani, R. W., Lestari, P. E., Setyorini, D., Jubhari, E. H., & Wibisono, S. (2019). Steeping from green and black robusta coffee beans increase viability of Peripheral Blood Mononuclear Cells (PBMC) and salivary leukocytes which is induced by streptococcus mutans. Journal of Dentomaxillofacial Science, 4(3), 154–158. https://doi.org/10.15562/jdmfs.v0i0.799
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I-Dewa A.R. Dewanti
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Roedy Budirahardjo
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Ristya WE. Yani
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Pujiana E. Lestari
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Dyah Setyorini
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Eri H. Jubhari
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Sunlip Wibisono
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