Original Article

Difference influence of rubbing and soaking tooth extraction instruments in 70% alcohol on total oral bacterial colonisation on clinical students at the Department of Oral Surgery and Maxilofacial March-May 2018

Ahyar Riza , Hendry Rusdy, Isnandar Isnandar, Evelin N. Sari

Ahyar Riza
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia. Email: ahyar.riza@usu.ac.id

Hendry Rusdy
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia

Isnandar Isnandar
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia

Evelin N. Sari
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
Online First: August 01, 2020 | Cite this Article
Riza, A., Rusdy, H., Isnandar, I., Sari, E. 2020. Difference influence of rubbing and soaking tooth extraction instruments in 70% alcohol on total oral bacterial colonisation on clinical students at the Department of Oral Surgery and Maxilofacial March-May 2018. Journal of Dentomaxillofacial Science 5(2): 74-76. DOI:10.15562/jdmfs.v0i0.815


Objective: To compare the effect of rubbing and soaking tooth extraction instrument in 70%  alcohol on total oral bacterial colonisation at the Department of Oral Surgery, Faculty of Dentistry, Universitas Sumatera Utara.

 

Material and Methods: This is an experimental research with a post-test only approach. The sampling technique used is Purposive Sampling. In this study, sample were divided into 2 groups consisting of 18 extraction instruments which are lower molar forceps rubbed with 70% alcohol and soaked in 70% alcohol. Each instrument were pre-cleaned using brush, water and soap prior disinfection process.

Results: The results were statistically analyzed using Mann-Whitney Test. The result showed no significant difference to the total bacteria count on rubbing and soaking using 70% alcohol.

Conclusion: Soaking in 70% alcohol method showed more effective because it overally kills bacteria.

Keyword: 

References

Suleh MM, Wowor VNS, Mintjelungan CN. Prevention and control of cross infection in the extraction of teeth at the Dental and Oral Hospital of PSPDG FK UNSRAT. J e-GIGI 2015;3: 588. (In Indonesia)

Reddy RV, Tanveer K, Sharma KD, et al. Evaluation of effectiveness of chemical disinfectants in reducing bacterial growth on ortodontic instruments. The J Contemp Dent Pract 2013;14: 1039.

Pankhurst CL, Coulter WA. Basic guide to infection prevention and control in dentistry. New Delhi: Aptara Inc; 2009. p. 1, 16-25, 93-95.

Susatyo JH. The difference in alcohol basting and immersion 70% in decreasing the number of germ count on dental instruments. J Vokasi Kesehatan 2016;2: 364-368.(In Indonesia)

Rutala WA, Weber DJ. Disinfection and sterilization: an overview. Am J Infect Control 2013;41: S3.

Muzhidah, Sukartini T, Sunarno A. The effectiveness of the combination of 0.5% chlorine and 70% alcohol on the growth of germs. J Ners 2009;4: 19-21. (In Indonesia)

Mulyanti S. Effect of 70% alcohol swabbing and flushing technique on high speed contra angle toward bacterial count and identification of pathogens. Padjajaran J Dentistry 2009;21: 71.

Keen JN, Austin M, Huang L, et al. Efficacy of soaking in 70% isopropyl alcohol on aerobic bacterial decontamination of surgical instruments and glove for serial mouse laparotomies. J Am Assoc for Lab Anim Sci 2010;49: 832.

Yunita M, Hendrawan Y, Yulianingsih R. Microbiological quantitative analysis of Garuda Indonesia aerofood ACS based on Total Plate Count (TPC) with the pour plate method. J Keteknikan Tropis dan Biosistem 2016;3: 237, 241-242. (In Indonesia)

Thomas P, Sekhar CA, Upreti R, et al. Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples. Biotechnol Reports J 2015;8: 48-49.

Riza A, Oes A, Rusdy H, et al. Comparison of the effectivity of handrubbing and handwashing on the number of bacterial colonization on clinical students at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara March-May 2018. J Dentomaxillofac Sci 2019;4: 105-108.


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