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Identification of active component in red pomegranate (punica granatum L.) seeds ethanolic extract


Objective: Pomegranate (punica granatum L.) have many benefits for health and used as traditional medicine. The pomegranate seeds are rich in antioxidants, namely flavonoids and tannins as well as alkaloid which is an antibacterial.  The aim of this study was to identify the pure isolate/active component of red pomegranate seeds ethanolic extract. Material and Methods: Two-ways TLC of the purified crystals which were obtained from the hexan fraction of ethanolic extract red pomegranate seeds, were used to make sure there was only 1 active compound of red pomegranate seeds. The isolate was identified using dragendorff, Mg and HCl, NaOH, Bunchard, Liebermen, FeCL3 and HCl concentrated reagents.Results: Isolate identification test using dragendorf reagents showed positive result (an orange-coloured).Conclusion: The pure isolate/active component of red pomegranate seeds is alkaloid.

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  1. Marhari OY, Dewi KK. Khasiat ajaib delima. 1st ed. Jakarta: Padi; 2014. p. 1-5, 23-24, 63.
  2. Narayan T, Deshpande S, Jha A, et al. Punica granatum (Pomegranate) fruit and its relevance in Oral Hygiene. J Dent Med Sci 2014;13: 29-34.
  3. Jurenka J. Therapeutic applications of pomegrante (punica granatum L.): a review. Alt Med Review 2008;13: 128-131, 137-138, 141.
  4. Gumienna M, Szwengiel A, Gorna, B. Bioactive components of pomegranate fruit and their transformation by fermentation processes. Eur Food Res Technol 2016;242: 631-640.
  5. Viuda-Martos M, Fernandez-Lopez J, Perez-Alvarez JA. Pomegranate and its many functional components as related to human health: a review. Comp Reviews Food Sci & Food Safety 2010;9: 635-654.
  6. Bhandari PR. Pomegranate (punica granatum L). Ancient seeds for modern cure? Review of potential therapeutic applications. Int J Nutr Pharm, Neurol Disea 2012;2: 171-184.
  7. Kumar KA, Vijayalakshmi K. In vitro anti-microbial activity and phytochemical analysis of selected fruit wastes. Int J Curr Microbiol App Sci 2013;2: 196-204.
  8. Rahmani AH, Alsahli MA, Almatroodi SA. Active constituents of pomegranates (punica granatum) as potential candidates in the management of health through modulation of biological activities. Pharm J 2017;9: 689-695.
  9. Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharm 2000;32: 81-118.
  10. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Reviews 1999;12: 564-582.
  11. Setiadhi R, Sufiawati I, Zakiawati D, et al. Inhibition growth of pomegranate seeds extract against streptococcus sanguis: the cause of recurrent aphthous stomatitis. J Dentomaxillofac Sci 2017;2: 7-11.
  12. Ajizah A. Sensitivitas salmonella typhimurium terhadap ekstrak daun psidium guajava L. Bioscient 2004;1: 31-38.
  13. Wullur AC, Schaduw J, Wardhani ANK. Identifikasi alkaloid pada daun sirsak (annona muricataL.). JIF 2012;3: 54-56.
  14. Pasaribu SP. Uji Boaktivitas Metabolit Sekunder dari Daun Tumbuhan Babandotan (Ageratum conyzoides L.). J Kimia Mulawarman 2009;6: 23-29.
  15. Riska A, Weny JA, Musa LaA. Identifikasi senyawa alkaloid dari ekstrak metanol kulit batang mangga (mangifera indica L). J Entropi 2013;8: 514-519.
  16. Olasupo AD, Aborisade AB, Olagoke OV. Phytochemical Analysis and Antibacterial Activities of Spinach Leaf. Am J Phytomedic Clin Therap 2018;2: 1-4.
  17. Narender B, Himabindu P. Phytochemical analysis and evaluation of in vitro antioxidant activity of punica granatum L. Int J Pharm Phytochem Res 2017;9; 1110-1118.
  18. Hammado N, Illing I. Identifikasi senyawa bahan aktif alkaloid pada tanaman lahuna (eupatorium odoratum). J Dinamika 2013;4: 1-18.
  19. Elaleem HGA, Albasheer AA, Elaleem KGA. Phytochemical screening and antibacterial activity of punica granatum fruit rind extracts. Glob J Med Plant Res 2016;4: 9-15.
  20. Jouvenez DP, Blum MMS, Maccconel JB. Antibacterial activity of fenom alkaloid from the imported fire and solepncsis invicta buren. Am Soc Microbiol 1972;2: 291-293.
  21. Karou D, Aly S, Antonella CS, et al. Antibacterial activity of fenom alkaloid from the imported fire and solepncsis invicta buren. Am Soc Microbiol 2006;2: 291-293.
  22. Kurniawan B, Aryana WF. Binahong (cassia alata L) as inhibitor of escherichia coli growth. J Majority 2015;4: 100-104.
  23. Darsana IGO, Besung INK, Mahatmi H. Potensi daun binahong (anredera cordifolia (tenore) steenis) dalam menghambat pertumbuhan bakteri escherichia coli secara in vitro. Ind Medic Veterinus 2012;1: 337-351.
  24. Cushnie TT, Cushnie B, Lamb AJ. Alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents 2015;44: 377-386.

How to Cite

Setiadhi, R., Sufiawati, I., & Hidayat, W. (2019). Identification of active component in red pomegranate (punica granatum L.) seeds ethanolic extract. Journal of Dentomaxillofacial Science, 4(2), 83–86.




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