Non-invasive early diagnosis of oral squamous cell carcinoma using piezoelectric biosensor
Abstract
Objective: To develop a device capable of distinguishing Oral squamous cell from other types of lesion so early diagnosis can be made non-invasively. Methods:The piezoelectric buzzer was coated with IgA EBV enzyme conjugate and antigen substance from Bionevan. The sensor was designed label free so further substance won’t be needed. The body of the device was made from white acrylic with 4mm thickness. The body was divided into 3 compartments each with different purpose. The main component consists of reagent coated piezoelectric buzzer and sensor. Both sensors are attached to Arduino UNO, breadboard, LIPO UBEC battery and LCD display network. The arduino UNO will read and process the difference between initial frequency and vibration frequency after the antigen were bind by the reagent, and shows the level of IgA in the LCD display.Results: The device worked and manage to show the level of IgA and presence of EBV in saliva but still need further improvement to make it more specific and validConclusion: Piezoelectric biosensor have shown promise to make early non-invasive diagnosis for OSCC
Section
References
- Gleber-Netto FM. Salivary biomarkers for detection of oral squamous cell carcinoma in a Taiwanese population. Clin Cancer Res 2016;1:OF1.
- Sathiyasekar AC, Chandrasekar P, Pakash A, et al.Overview of immunology of oral squamous cell carcinoma. J Pharm Bioallied Sci 2016;8: S8.
- Ajila V, Shetty V, Babu S, et al. Immunoglobulin a in oral potentially malignant disorders and oral squamous cell carcinoma. J Med Sci 2017;37:195-197.
- Radhika T, Jeddy S, Nithya RM, et al. Salivary biomarkers in oral squamous cell carcinoma – An insight. J Oral Bio Craniofacial Res 2016;6: S51.
- Malon RSP, Sadir D, Balakrishnan M, et al. Saliva-based biosensors: noninvasive monitoring tool for clinical diagnostics. BioMed Res Int 2014;1:1,12.
- Javaid MA, Ahmed AS, Durand R, et al. Saliva as a diagnostic tool for oral and systemic diseases. J Oral Bio Craniofacial Res 2016;6: 66-67.
- Wulansari DP, Arsyad A, Rieuwpassa IE, Hamrun N, Hasyim R. Analysis of genotoxic and cytotoxic effects of oral mucosa in smokers and non-smokers after exposed to digital intraoral radiography. J Dentomaxillofac Sci 2019;4(3):159.
- Liu Y, Li Y, Chang R, et al. Identification of proteinaceous binders in Ancient Tripitaka by the use of an enzyme-linked immunosorbent assay. Anal Sci 2016;32: 735.
- Thiha A, Ibrahim F. A Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) Detection Platform for a Point-of-Care Dengue Detection System on a Lab-on-Compact-Disc. Sensors 2015;15: 11431.
- Malhotra S, Verma A, Tyagi N, et al. Biosensors: principle, types, and applications. IJIARIIE 2017;3: 3639.
- Pohanka M. Overview of piezoelectric biosensors, immunosensors and DNA sensors and their applications. Materials (Basel) 2018;11: 1-4.
- Pohanka M. The piezoelectric biosensors: principles, applications, a review. Int J Electrochem Sci 2017;12: 496-497.
- Saluja RM Kale A, Hallikerimath S, Determination of levels of salivary IgA subclasses in patients with minor recurrent apthous ulcer. J Oral Maxillofac Pathol 2012;16: 50.
- Mohammad R, Halboub E, Mashlah A, et al. Levels of salivary IgA in patients with minor recurrent apthous stomatitis: a matched case-control study. Clin Oral Investig 2013;17: 975.
How to Cite
Rieuwpassa, I. E., Sumintarti, Kim, Y.-R., Matandung, M., & Matongan, G. S. (2020). Non-invasive early diagnosis of oral squamous cell carcinoma using piezoelectric biosensor. Journal of Dentomaxillofacial Science, 5(3), 146–149. https://doi.org/10.15562/jdmfs.v0i0.942
