Original Article

Analysis of genotoxic and cytotoxic effects of oral mucosa in smokers and non-smokers after exposed to digital intraoral radiography

Dwi P. Wulansari , Aryadi Arsyad, Irene E. Rieuwpassa, Nurlindah Hamrun, Rafikah Hasyim

Dwi P. Wulansari
Department of Dental Radiology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Email: dpw.dentalradiologyunhas@gmail.com

Aryadi Arsyad
Department of Micro, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

Irene E. Rieuwpassa
Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia

Nurlindah Hamrun
Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia

Rafikah Hasyim
Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Online First: December 01, 2019 | Cite this Article
Wulansari, D., Arsyad, A., Rieuwpassa, I., Hamrun, N., Hasyim, R. 2019. Analysis of genotoxic and cytotoxic effects of oral mucosa in smokers and non-smokers after exposed to digital intraoral radiography. Journal of Dentomaxillofacial Science 4(3): 159-162. DOI:10.15562/jdmfs.v4i3.961

Objectives : To analyze genotoxic and sitotoxic indicators of buccal epithelial cells by measuring the number of cells contain micronucleus, pycnosis, karyorrhexis, karyolysis and to determine the change in oxidative stress of  oral mucosa cells using salivary MDA levels between smokers and nonsmokers before and after exposure to intraoral radiographs during endodontic treatment.

Material and Methods : This research was an analytic observation research with cross-sectional design. The sample was divided into smokers (n = 5) and non-smokers group (n = 5). The buccal epithelial cells was taken with cytobrush and unstimulated saliva in each group were taken by the draining method shortly before intraoral radiography, 10±2 days after first  day exposure, 10±2 days after second day exposure. The cells were stained using Papanicolau (PAP) kit and observed by pathologist. Genotoxic and cytotoxic indicator measured by counted the number of cells contain mironucleus, pyknosis, karyorrhexis and karyolysis under light microscope with 400X magnification according to the Tolbert criteria in 1000 cells. While oxidative stress of cell measured by salivary MDA with Thiobarbitoric Acid (TBA) assay.

Result : The number of micronucleus, pyknosis, karyorrhexis, karyolysis in buccal epithelial cells and salivary MDA levels in smokers was higher than non-smokers (p<0,05). There were no significant differences in genotoxic and cytotoxic indicators and salivary MDA levels before and after the last exposure in smokers and non-smokers (p>0,05).

Conclusion : The results of this study indicate that the use of intraoral radiographs is within safe limits despite repeated exposure after several days.


White SC, Pharoah MJ. Oral radiology-E-Book: Principles

and interpretation. Elsevier Health Sciences; 2014.

Abbas AW, Zaidan TF, Al-barrak AYA. Assessment of

serum and salivary malondialdehyde in patients with oral

lichen planus. J Bagh Coll Dent 2014;26: 99-102.

Amtha R, Kurniadi A. An overview of oral mucosa

condition of shisha smoker. J Dentomaxillofacial Sci

;1: 67-70.

Gowhar O. Comparison of exfoliative cytology of tongue

and buccal mucosa among smokers and non-smokers

using PAP stain and AgNOR counts. Int J Contemp Med

Res 2017;4: 1587-1590.

Kusuma A. alveolar bone loss, tooth loss. J UNISSULA

;1: 1-8.

Khoubnasabjafari M, Ansarin K, Jouyban A. Salivary

malondialdehyde as an oxidative stress biomarker in

oral and systemic diseases. J Dent Res Dent Clin Dent

Prospects 2016;10: 71-75.

Cerqueira EMM, Gomes-filho IS, Trindade S, et al.

Genetic damage in exfoliated cells from oral mucosa of

individuals exposed to x-rays during panoramic dental

radiographies. Mutat Res 2004;562: 111-117.

Angelieri F, Yujra VQ, Tizuko C, et al. Do Dental x-rays

induce genotoxicity and cytotoxicity in oral mucosa cells?

a critical review. Anticancer Res 2017; 37: 5383-5388.

Kesidi S, Maloth KN, Vinay K, et al. Genotoxic and

cytotoxic biomonitoring in patients exposed to full

mouth radiographs – a radiological and cytological study.

J Oral Maxillofac Radiol 2017;5: 1-6.

Smriti K, Muralidhar K, Ravindranath V. Science direct

role of salivary malondialdehyde in assessment of oxidative stress among diabetics. J Oral Biol Craniofacial Res

;6: 41-44.

Panganiban RM, Snow AL, Day RM. Mechanisms of

radiation toxicity in transformed and. Int J Mol Sci

;14: 15931-15958.

Rahmah N. Analisis Sitogenik Mukronukleus Mukosa

Bukal pada Perokok Aktif dan Pasif. Dentino

;1: 15-20.

Holland N, Bolognesi C, Kirsch-volders M, et al.

Mutation research/reviews in mutation research the

micronucleus assay in human buccal cells as a tool for

biomonitoring DNA damage : The HUMN project

perspective on current status and knowledge gaps. Mutat

Res 2008;659: 93-108.

Ozturk O, Meydan A, Healthcare F. Effects of

smoking on oral cavity. J Exp Clin Med 2017;34: 3-7.

Fenech M, Natarajan AT. Molecular mechanisms of

micronucleus, nucleoplasmic bridge and nuclear bud

formation in mammalian and human cells. Mutagenesis

;26: 125-132.

Demİrtaş M, Şenel Ü, Yüksel S, et al. A comparison of the

generation of free radicals in saliva of active and passive

smokers. Turkish J Med Sci 2014;44: 208-211.

Menicagli R, Duca MRP. Development research

monitoring of oxidative stress induced by cigarette

smoke by analysis salivary malondialdehide with the use

of dipsticks. Int J Dev Res 2016;6: 7546-7551.

Kurku H, Kacmaz M, Kisa U, et al. Acute and chronic

impact of smoking on salivary and serum total

antioxidant. J Pak Med Assoc 2015;65: 164-169.

Li G, Yang P, Hao S, et al. Buccal mucosa cell damage in

individuals following dental x-ray examinations. Sci Rep.

;1: 1-7.

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