Skip to main content Skip to main navigation menu Skip to site footer

The effectiveness of proanthocyanidins cacao pods (theobroma cacao l) on increasing socket epithelial thickness post toothextraction in wistar rats

Abstract

Objective: The aim of this study was examine theeffectivenessof proanthocyanidins cacaopods (Theobroma cacao L) extract on increasing socketepithelialthicknessposttooth extractionin Wistar Rats. Proanthocyanidins has benefis as antiinflammation, antioxidant and antibacteria agent. Post-extraction wound can cause complication, which can make long the healing process. This is where anti-inflammatory is needed to prevent infection, so that it can avoid bacterial contamination and can shorten the inflammatory process. Material and Methods:These study was an experimental laboratory research on thickness of socket epithelium of Wistar rats given proanthocyanidins cacao pod extract post extraction. A total of 24 male Wistar rats was divided into 3 group; which was treated with 100 mg/ml proanthocyanidins cocoa pod extract as treatment group, distilled placebo gel as negative control group and alvogyl as positive control group. On day 7 and day 14 the sampel was sacrified and tissue processing was made. The gingival epithelial thickness was determined by software image raster on light microscopy. Results:Analysisof One way Anova showed there was a significant difference0.001 between groups. The epithelium oftreatment group, was thicker than the control group significantly.Conclusion:Proanthocyanidins cocoa pods (Theobroma cacao L)  extract effectiveincreasing the thickness of socket epithelium postextraction in Wistar rats.
Section

References

  1. Ardhana MM, Fleet HG. The microbial ecology of cocoa bean fermentations in Indonesia. Int J Food Microbiol 2003;86: 87-99.
  2. Smeriglio A, Barreca D, Bellocco E, et al. Proanthocyanidins and hydrolysable tannins: occurrence, dietary intake and pharmacological effects. Br J Pharmacol 2017;174: 1244-1262.
  3. Hii CL, Law CL, Suzannah S, et al. Polyphenols in cocoa (theobroma cacao L.). As J Food Ag-Ind 2009;2: 702-722
  4. Park M, Cho H, Jung H, et al. Antioxidant and anti-inflammatory activities of tannin fraction of the extract from black raspberry seeds compared to grape seeds. J Food Biochem 2014;38: 259-270.
  5. Huang Q, Liu X, Zhao G et al. Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production.AnimNutr.2018;4:137-150.
  6. Del-Fabbro M, Bucchi C, Lolato A, et al. Healing of postextraction sockets preserved with autologous platelet concentrates. a systematic review and meta-analysis. J Oral Maxillofac Surg 2017;75: 1601-1615.
  7. Zakia F, Novita CF, Sunnati. DistribusiFrekuensiFaktorPenyebabEkstraksi Gigi PasienDi Rumah Sakit Umum dr. Zainoel Abidin Banda Aceh Periode Mei-Juli 2016. J Caninus Dentist 2016;1: 32-38.
  8. Leoni G,Neumann PA,Sumagin R, et al. Wound repair: role of immune–epithelial interactions. Mucosal Immunol 2015;8: 959-968.
  9. Pastar I, Stojadinovic O, Yin NC. Epithelialization in wound healing: a comprehensive review. Adv Wound Care (New Rochelle). 2014;3: 445-464.
  10. Baharudin M. The antiinflammatory test of cocoa pods (theobroma cacao l.) extractonmale mice. Sumatra Utara: Universitas Sumatra Utara;1996.
  11. Maulina, L, Sugihartini, N. Formulation of ethanol mangosteen pods gel extract (garcinia mangostana l.) with variation of gelling agent for preparation of burns. Pharmaciana 2015;5: 43-52.
  12. Wissam Z, Bashour G, Abdelwahed W, et al. Simple and fast method for the extraction of polyphenol and the separation of proanthocyanidins from carob pods. SAJP 2013;2: 375-380.
  13. Qiang LV, Luo1 F, Zhao X, et al. Identification of proanthocyanidins from litchi (litchi chinensissonn.) pulp by LC-ESIQ-TOF-MS and their antioxidant activity. Plos One 2015;10: 1-17.
  14. Hafidhah N, Hakim RF, Fakhurranzi. The effect of cacao seed extract in different concentration on growth of Enterococcus faecalis. J Canalis Dentist 2017;2: 341-343. (In Indonesian, English abstract).
  15. Yuwono B, Elga S, Christmarini DM. The potential of cacao (theobroma cacao l.) pod husk extract on the number of fibroblast cells of wistar rats wound post-tooth extraction. Int J App Pharm 2019;11: 30-32.
  16. Simon BI, Zatcoff AL, Kong JJW, et al. Clinical and histological comparison of extraction socket healing following the use of autologous platelet-rich fibrin matrix (PRFM) to ridge preservation procedures employing demineralized freeze dried bone allograft material and membrane. Open Dent J 2009;3: 92-99.
  17. Nagar HK, Srivastava AK, Srivastava R, et al. Pharmacological investigation of the wound healing activity of cestrum nocturnum (L.) ointment in wistar albino rats. J Pharm (Cairo) 2016;2016: 9249040.
  18. Setyorini D, Rahayu YC, Sistyaningrum T. The effects of rinsing red beet root (Beta vulgaris L.) juice on streptococcus sp. dental plaque. J Dentomaxillofac Sci 2017;2: 15-17.
  19. Sutcliffe TC, Winter AN, Punessen NC, et al. Procyanidin B2 Protects Neurons from Oxidative, Nitrosative, and Excitotoxic Stress. Antioxidants 2017;6: 77.

How to Cite

Rahayu, Y. C., Yuwono, B., & Wandansari, A. (2020). The effectiveness of proanthocyanidins cacao pods (theobroma cacao l) on increasing socket epithelial thickness post toothextraction in wistar rats. Journal of Dentomaxillofacial Science, 5(1), 12–16. https://doi.org/10.15562/jdmfs.v5i1.963

HTML
491

Total
519

Share

Search Panel