Original Article

Indonesian propolis suppressed the expression of COX-2 in inflamed rat dental pulp in direct capping treatment

Ardo Sabir

Ardo Sabir
Department of Conservative Dentistry, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia. Email: ardo.sabir@yahoo.com
Online First: August 01, 2019 | Cite this Article
Sabir, A. 2019. Indonesian propolis suppressed the expression of COX-2 in inflamed rat dental pulp in direct capping treatment. Journal of Dentomaxillofacial Science 4(2): 109-113. DOI:10.15562/jdmfs.v4i2.966


Objective: The aim of the present study ass to analysis the expression COX-2 on inflamed rat dental pulp after capped with propolis.

Material and Methods: Propolis was obtained from South Sulawesi, Indonesia. Flavonoid and non-flavonoid were purified from an ethanolic extract of propolis (EEP). Eighty male Sprague Dawley rats were divided randomly into 5 groups, each group consisting of 16 rats. Group I as a negative control, rats were left without any treatment. A Class I cavity was prepared on the occlusal surface of permanent maxillary right first molar. Dental pulp was exposed and allowed in oral environment for 1 hour, then capping with EEP (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide (Ca(OH)2) (group V). All cavities were filled with glass ionomer cement. The animals were sacrificed at 6 hours, 2, 4 or 7 days after treatment, biopsy samples were obtained, processed for immunostaining of COX-2 and viewed under light microscope. Data was statistically analyzed using Friedman and Kruskal-Wallis tests with significant level P<0.05.

Results: Except in group I, COX-2 expression was decreased in all treatment group with the longer of observation time periods. In group II and group V, COX-2 expression was weaker than group III and IV at all periods time. However, there was no statistically significant differences of COX-2 expression among groups for each time period.

Conclusions: The present study suggests that EEP and Ca(OH)2 were stronger than other materials test to suppressed COX-2 expression on inflamed rat dental pulp.

References

Kuropatnicki AK, Szliszka E, Krol W. Historical aspects

of propolis research in modern times. Evid Based

Complement Alternat Med 2013;2013: 964149.

Simone-Finstrom M, Borba RS, Wilson M, et al. Propolis

counteracts some threats to honey bee health. Insects 2017;8:

E46.

Pietta PG, Gardana C, Pietta AM. Analytical methods for

quality control of propolis. Fitoterapia. 2002;73 :S7-20.

Sforcin JM, Fernandes A Jr, Lopes CA, et al. Seasonal

effect on Brazilian propolis antibacterial activity. J

Ethnopharmacol 2000;73: 243-249.

Wang K, Zhang J, Ping S, et al. Anti-inflammatory

effects of ethanol extracts of Chinese propolis and buds

from poplar (Populus x canadensis). J Ethnopharmacol

;155: 300-311.

Sabir A, Mooduto L, Kaelan C, et al. Impact of the use

of ethanolic extract of propolis, flavonoid and nonflavonoid

propolis for direct pulp capping in collagen

type I density. Braz J Oral Sci 2016;15: 264-268.

Sforcin JM. Propolis and the immune system: a review.

J Ethnopharmacol 2007;113: 1-14.

Veloz JJ, Saavedra N, Alvear M, et al. Polyphenol-rich

extract from propolis reduces the expression and

activity of Streptococcus mutans glucosyltransferases at

subinhibitory concentrations. Biomed Res Int 2016;2016:

Sabir A, Tabbu CR, Agustiono P, et al. Histological

analysis of rat dental pulp tissue capped with propolis.

J Oral Sci 2005;47: 135-138.

Toreti VC, Sato HH, Pastore GM, et al. Recent progress

of propolis for its biological and chemical compositions

and its botanical origin. Evid Based Complement Alternat

Med 2013;2013: 697390.

Huang S, Zhang CP, Wang K, et al. Recent advances

in the chemical composition of propolis. Molecules

;19: 19610-19632.

Anauate NC, Marcucci MC, Paulino N, et al. Effects of

typified propolis on mutans streptococci and lactobacilli:

a randomized clinical trial. Braz Dent Sci 2013;16: 31-36.

Purra AR, Mushtaq M, Acharya SR, et al. A comparative

evaluation of propolis and 5.0% potassium nitrate as a

dentine desensitizer: a clinical study. J Indian Soc

Periodontol 2014;18: 466-471.

Bhardwaj A, Velmurugan N, Sumitha, et al. Efficacy of

passive ultrasonic irrigation with natural irrigants

(morinda citrifolia juice, aloe vera and propolis) in

comparison with 1% sodium hypochlorite for removal

of E. faecalis biofilm: An in vitro study. Indian J Dent

Res 2013;24: 35-41.

Prabhakar AR, Karuna YM, Yavagal C, et al. Cavity

disinfection in minimally invasive dentistry-comparative

evaluation of Aloe vera and propolis: a randomized

clinical trial. Contemp Clin Dent 2015;6: S24-31.

Bazvand L, Aminozarbian MG, Farhad A, et al.

Antibacterial effect of triantibiotic mixture, chlorhexidine

gel, and two natural materials propolis and aloe vera

against enterococcus faecalis: an ex vivo study. Dent

Res J (Isfahan) 2014;11: 469-474.

Parolia A, Kundabala M, Rao NN, et al. A comparative

histological analysis of human pulp following direct

pulp capping with propolis, mineral trioxide aggregate

and Dycal. Aust Dent J 2010;55: 59-64.

Al-Shaher A, Wallace J, Agarwal S, et al. Effect of

propolis on human fibroblasts from the pulp and

periodontal ligament. J Endod 2004;30: 359-361.

Zare JM, Ranjbarian P, Shiravi S. Cytotoxicity evaluation

of Iranian propolis and calcium hydroxide on dental

pulp fibroblasts. J Dent Res Dent Clin Dent Prospects

;8: 130-133

Mejàre IA, Axelsson S, Davidson T, et al. Diagnosis

of the condition of the dental pulp: a systemic review.

Int Endod J 2012;45: 597-613.

Needleman P, Turk J, Jakschik BA, et al. Arachidonic

acid metabolism. Annu Rev Biochem 1986;55: 69-102.

Holt CI, Hutchins MO, Pileggi R. A real time quantitative

PCR analysis and correlation of COX-1 and COX-2

enzymes in inflamed dental pulps following administration

of three different NSAIDs. J Endod. 2005;31:799-804.

Nakanishi T, Shimizu H, Hosokawa Y, et al. An

immunohistological study on cyclooxygenase-2 in human

dental pulp. J Endod 2001;27: 385-388.

Güven G, Altun C, Günhan Ö, et al. Co-expression of

cyclooxygenase-2 and vascular endothelial growth factor

in inflamed human pulp: an immunohistochemical

study. J Endod. 2007;33: 18-20.

Faleiro-Rodrigues C, Macedo-Pinto I, Pereira D, et al.

Association of E-cadherin and beta-catenin immunoexpression

with clinicopathologic features in primary

ovarian carcinomas. Hum Pathol 2004;35: 663-669.

Chang YC, Yang SF, Huang FM, et al. Proinflammatory

cytokines induce cyclooxygenase-2 mRNA and protein

expression in human pulp cell cultures. J Endod 2003;29:

-204.

Chang MC, Chen YJ, Tai TF, et al. Cytokine-induced

prostaglandin E2 production and cyclooxygenase-2

expression in dental pulp cells: downstream calcium

signaling via activation of prostaglandin EP receptor.

Int Endod J 2006;39: 819-826.

Lin SK, Kuo MY, Wang JS, et al. Differential regulation

of interleukin-6 and inducible cyclooxygenase gene

expression by cytokines through prostaglandin-dependent

and-independent mechanisms in human dental pulp

fibroblasts. J Endod 2002;28: 197-201.

Tsai CH, Huang FM, Yang LC, et al. Immunohistochemical

localization of cyclooxygenase-2 in radicular

cysts. Int Endod J 2002;35: 854-858.

Paulino M, Alvareda E, Iribarne F, et al. Toward the

understanding of the molecular basis for the inhibition

of COX-1 and COX-2 by phenolic compounds present

in Uruguayan propolis and grape pomace. J Biomol

Struct Dyn 2016;34: 2643-2657.

Sabir A, Sumidarti A. Interleukin-6 expression on

inflamed rat dental pulp tissue after capped with

Trigona sp. propolis from South Sulawesi, Indonesia.

Saudi J Biol Sci 2017; 24: 1034-1037.

Chirumbolo S. Anti-inflammatory property of propolis.

J Clin Biochem Nutr 2015;56: 163-164.

Rossi A, Ligresti A, Longo R, et al. The inhibitory effect of

propolis and caffeic acid phenethyl ester on cyclooxygenase

activity in J774 macrophages. Phytomedicine 2002;9:

-535.

Fransson H. On the repair of the dentine barrier. Swed

Dent J Suppl 2012;226: 9-84.

Nelson-Filho P, Silva LA, Leonardo MR, et al. Connective

tissue responses to calcium hydroxide-based root canal

medicaments. Int Endod J 1999;32: 303-311.


Article Views      : 69
PDF Downloads : 60

Related Articles

Search Authors in












  • Ardo Sabir

  • Ardo Sabir

  • Ardo Sabir