Original Article

Analysis of gene polymorphisms TGFα rs 1058213 to cleft lip cases with or without a non-syndrome sky cleft in Riau Malay Ethnic

Masrial Masrial , Yanwirasti Yanwirasti, Sumaryati Syukur, Endang Syamsudin

Masrial Masrial
Department of Biomedicine, Faculty of Medicine, Andalas University, Padang, Indonesia. Email: drg.masrial.spbm@gmail.com

Yanwirasti Yanwirasti
Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia

Sumaryati Syukur
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Padang, Indonesia

Endang Syamsudin
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia
Online First: April 01, 2018 | Cite this Article
Masrial, M., Yanwirasti, Y., Syukur, S., Syamsudin, E. 2018. Analysis of gene polymorphisms TGFα rs 1058213 to cleft lip cases with or without a non-syndrome sky cleft in Riau Malay Ethnic. Journal of Dentomaxillofacial Science 3(1): 32-36. DOI:10.15562/jdmfs.v3i1.498


Objective: This study aims to determine the role of gene polymorphism TGFα rs 1058213 in cases of cleft lip with or without cleft palate non-syndrome in the Riau Malay tribe.

Material and Methods: DNA extraction of venous blood and polymorphism TGFα rs 1058213 was assessed using the Restriction Fragment Length Polymorphism-PCR (RFLP-PCR) working method and followed by sequencing. Genotyping SNP rs1058213 in the TGFα gene was performed by the RFLP-PCR method in control and CLP NS patients. The data was assessed using the test statistic premises T-test difference test.

Results: The total sample size included 68 samples consisting of 34 CLP NS samples and 34 control samples. The cases of CLP NS occurred more in men than women, 20 cases (59%) and 14 cases (14%), respectively.

Conclusion: There was no significant difference between TGFα rs 1058213 gene polymorphisms in CLP NS and controls in the Riau Malay tribe.

References

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Mossey PA, Little J, Munger RG, et al. Cleft lip and palate. Lancet 2009;374: 1773-1785.

Malik NA. Textbook of oral and maxillofacial surgery. JP Medical Ltd; 2012. p. 615-618.

Sull JW, Liang KY, Hetmanski JB, et al. Evidence that TGFA influences risk to cleft lip with/without cleft palate through unconventional genetic mechanisms. Human Genetics 2009;126: 385-394.

Lidral AC, Moreno LM. Progress toward discerning the genetics of cleft lip. Current Opinion Pediatrics 2005;17: 731.

Rajion ZA, Alwi Z. Genetics of cleft lip and palate: a review. Malay J Med Sci 2007;14: 4-9.


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